Abstract
A total of 126 Chinese sugar beet breeding lines were evaluated for their fertility gene composition using molecular marker-assisted selection (MAS). The identification of male-sterile cytoplasm (S) and normal cytoplasm (N) using the TR1 locus and the composition of the Rf1 and Rf2 fertility genes were analyzed using the molecular markers s17 and o7. N and S cytoplasm were distinguished by the variable number of tandem repeat (VNTR) amplification and showed that 25.4% of the lines displayed N cytoplasm, 33.33% S cytoplasm, and the remaining 41.27% mixed cytoplasm. Results using the s17 showed the presence of 1800 bp bands in 77 lines while enzymatic digestion showed that 72 lines displayed pattern 5/5, one line displayed pattern 4/4, and seven lines displayed pattern 4/5, with the remaining bands all being mixed. Analysis using the o7 identified four lines with 2600 bp bands, 25 lines with 1400 bp bands, and 85 lines with mixed bands. No maintainer lines were discovered in the materials, and the majority of the materials displayed mixed genotypes to varying degrees, shown by a comprehensive analysis of cytoplasmic and nuclear fertility-related genetic markers. These findings indicated the presence of numerous non-O-type lines among the sugar beet lines in China.
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This work was supported by the Special Fund for the Improvement of High-quality Sugar Beet Varieties of the National Sugar Modern Agricultural Industrial Technology System (CARS-170111).
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Peng, F., Li, S., Pi, Z. et al. Identification of Molecular Markers for Fertility in Sugar Beet. Sugar Tech 25, 1167–1172 (2023). https://doi.org/10.1007/s12355-023-01275-y
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DOI: https://doi.org/10.1007/s12355-023-01275-y