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Screening of Polymorphic SSR Molecular Markers Between Resistant and Susceptible Parents for Localization of Brown Rust Resistance Gene

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Abstract

Sugarcane brown rust induced by Puccinia melanocephala is an important global disease. Exploring novel resistance genes and breeding varieties with durable resistance is the most economical and effective way of controlling brown rust. The establishment of complete and high-density molecular genetics maps is a key prerequisite for the discovery and location of sugarcane brown rust resistance genes. To obtain more polymorphic simple sequence repeat (SSR) markers for the construction of genetic linkage maps, six highly resistant varieties and six highly susceptible varieties were used as parents to screen the primers with clear bands, obvious polymorphism, and good repeatability. The highest numbers of polymorphic primers were obtained from the parental group of Liucheng 03-1137 × Dezhe 93-88 (52.38%). Ten pairs of primers including mSSCIR34 exhibited high polymorphism in the parental group of Liucheng 03-1137 × Dezhe 93-88. Here, we recommend ten SSR pairs of polymorphic primers including mSSCIR34 to use in the map** populations of Liucheng 03-1137 × Dezhe 93-88. The polymorphic primers could facilitate the construction of genetic maps and lay an appropriate foundation for the localization of brown rust resistance genes and development of molecular genetics linkage markers.

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Acknowledgements

This research was conducted with financial support from National Natural Science Foundation of China (31660419), Sugar Crop Research System (CARS-170303), Yunling industry and technology leading talent training program “Control of Sugarcane Harmful Organisms” (2018LJRC56) and an Earmarked Fund from Yunnan Province Agriculture Research System.

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  1. Hongli Shan and Wenfeng Li have contributed equally to this work.

Authors and Affiliations

  1. Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, 661699, Yunnan Province, China

    Hongli Shan, Wenfeng Li, Yingkun Huang, **aoyan Wang, Rongyue Zhang, Jie Li, Zhiming Luo, Jiong Yin & **aoyan Cang

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  1. Hongli Shan
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  2. Wenfeng Li
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Contributions

WL, YH, and HS designed the experiments and markers. ZL, JY, and XC collected parental materials, while HS, XW, RZ, and LJ performed the experiments. HS, XW, and RZ analyzed the data. HS and WL wrote the article. WL and YH revised the article.

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Correspondence to Yingkun Huang.

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Shan, H., Li, W., Huang, Y. et al. Screening of Polymorphic SSR Molecular Markers Between Resistant and Susceptible Parents for Localization of Brown Rust Resistance Gene. Sugar Tech 22, 1–7 (2020). https://doi.org/10.1007/s12355-019-00750-9

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  • DOI: https://doi.org/10.1007/s12355-019-00750-9

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