Abstract
DNA methylation is the most important epigenetic modification involved in many essential biological processes. MET1 is one of DNA methyltransferases that affect the level of methylation in the entire genome. To explore the effect of MET1 gene silencing on gene expression profile of Chrysanthemum × morifolium 'Zi**gling'. The stem section and leaves at the young stage were taken for transcriptome sequencing. MET1-RNAi leaves had 8 differentially expressed genes while 156 differentially expressed genes were observed in MET1-RNAi stem compared with control leaves and stem. These genes encode many key proteins in plant biological processes, such as transcription factors, signal transduction mechanisms, secondary metabolite synthesis, transport and catabolism and interaction. In general, 34.58% of the differentially expressed genes in leaves and stems were affected by the reduction of the MET1 gene. The differentially expressed genes in stem and leaves of transgenic plants went through significant changes. We found adequate amount of candidate genes associated with flowering, however, the number of genes with significant differences between transgenic and control lines was not too high. Several flowering related genes were screened out for gene expression verification and all of them were obseved as consistent with transcriptome data. These candidate genes may play important role in flowering variation of chrysanthemum. This study reveals the mechanism of CmMET1 interference on the growth and development of chrysanthemum at the transcriptional level, which provides the basis for further research on the epigenetic regulation mechanism in flower induction and development.
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File contains detailed descriptions of all supplemental files. Transcriptomic data are available at NCBI with the accession number: SUB7902302. Gene sequences are available upon request. The authors affirm that all data necessary for confirming the conclusions of the article are present within the article, figures and tables.
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This research was funded by National Key Research and Development Program (2018YFD1000403), National Natural Science Foundation of China (31372090) and Science and Technology Development Project of Henan (182300410026).
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L.S.L\ZY performed experiments and helped in data analysis. LSL and ZY analyzed data. KDR wrote the manuscript with contributions from all authors. WZC guided the design of the whole test scheme. All authors have read and approved the final manuscript.
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Supplementary Materials: Table S1. Sequences for fluorescent quantitative. Table S2. Overview of the sequencing and assembly. Table S3. Length distribution of the transcripts and unigenes. Table S4. Functional annotation of unigenes. Table S5. Details of 122 different gene annotation results.
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Kang, Dr., Zhu, Y., Li, Sl. et al. Transcriptome analysis of differentially expressed genes in chrysanthemum MET1 RNA interference lines. Physiol Mol Biol Plants 27, 1455–1468 (2021). https://doi.org/10.1007/s12298-021-01022-1
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DOI: https://doi.org/10.1007/s12298-021-01022-1