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Regulation of iron homeostasis by peroxide-sensitive CatR, a Fur-family regulator in Streptomyces coelicolor

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

CatR, a peroxide-sensing transcriptional repressor of Fur family, can de-repress the transcription of the catA gene encoding catalase upon peroxide stress in Streptomyces coelicolor. Since CatR-regulated genes other than catA and its own gene catR have not been identified in detail, the understanding of the role of CatR regulon is very limited. In this study, we performed transcriptomic analysis to identify genes influenced by both ΔcatR mutation and hydrogen peroxide treatment. Through ChIP-qPCR and other analyses, a new consensus sequence was found in CatR-responsive promoter region of catR gene and catA operon for direct regulation. In addition, vtlA (SCO2027) and SCO4983 were identified as new members of the CatR regulon. Expression levels of iron uptake genes were reduced by hydrogen peroxide and a DmdR1 binding sequence was identified in promoters of these genes. The increase in free iron by hydrogen peroxide was thought to suppress the iron import system by DmdR1. A putative exporter protein VtlA regulated by CatR appeared to reduce intracellular iron to prevent oxidative stress. The name vtlA (VIT1-like transporter) was proposed for iron homeostasis related gene SCO2027.

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Acknowledgements

This research was supported by a grant (2018R1D1A1B0704-6585 to K.-L.L and 2020R1I1A1A01052942 to Y.-J.C) of the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Republic of Korea. This work was also supported by a grant (2017R1A2A1A05000735 to J.-H.R.) of the NRF funded by the Ministry of Education, Science, and Technology (MEST), Republic of Korea. Y.K. was supported by B.K. Plus Fellowship for Biological Sciences at Seoul National University.

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Authors and Affiliations

  1. Department of Biological Sciences, Seoul National University, Seoul, 08826, Republic of Korea

    Yeonbum Kim, Jung-Hye Roe, Joo-Hong Park & Yong-Joon Cho

  2. The Research Institute of Basic Sciences, Seoul National University, Seoul, 08826, Republic of Korea

    Yong-Joon Cho

  3. Department of Biology Education, IALS, Gyeongsang National University, **ju, 52828, Republic of Korea

    Kang-Lok Lee

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  1. Yeonbum Kim
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  4. Yong-Joon Cho
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Contributions

YK and KL design the experiments. YK performed the experiments. YK, YC, KL, and JR analyzed and interpreted the data. YK, YC, JP, KL, and JR prepared the manuscript. YK, YC and KL wrote the manuscript. All authors reviewed the manuscript.

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Correspondence to Yong-Joon Cho or Kang-Lok Lee.

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Kim, Y., Roe, JH., Park, JH. et al. Regulation of iron homeostasis by peroxide-sensitive CatR, a Fur-family regulator in Streptomyces coelicolor. J Microbiol. 59, 1083–1091 (2021). https://doi.org/10.1007/s12275-021-1457-1

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  • DOI: https://doi.org/10.1007/s12275-021-1457-1

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