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Spirosoma montaniterrae sp. nov., an ultraviolet and gamma radiation-resistant bacterium isolated from mountain soil

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

A Gram-negative, yellow-pigmented, long-rod shaped bacterial strain designated DY10T was isolated from a soil sample collected at Mt. Deogyusan, Jeonbuk province, South Korea. Optimum growth observed at 30°C and pH 7. No growth was observed above 1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain DY10T belonged to the genus Spirosoma and was distantly related to Spirosoma arcticum R2–35T (91.0%), Spirosoma lingual DSM 74T (90.8%), Spirosoma endophyticum EX36T (90.7%), Spirosoma panaciterrae DSM 21099T (90.5%), Spirosoma rigui WPCB118T (90.2%), Spirosoma spitsbergense DSM 19989T (89.8%), Spirosoma luteum DSM 19990T (89.6%), Spirosoma oryzae RHs22T (89.6%), and Spirosoma radiotolerans DG5AT (89.1%). Strain DY10T showed resistance to gamma and ultraviolet radiation. The chemotaxonomic characteristics of strain DY10T were consistent with those of the genus Spirosoma, with the quinone system with MK-7 as the predominant menaquinone, iso-C15:0, C16:1 ω5c, and summed feature3 (C16:1 ω7c/C16:1 ω6c), and phosphatidylethanolamine as the major polar lipid. The G+C content of the genomic DNA was 53.0 mol%. Differential phenotypic properties with the closely related type strains clearly distinguished strain DY10T from previously described members of the genus Spirosoma and represents a novel species in this genus, for which the name Spirosoma montaniterrae sp. nov. is proposed. The type strain is DY10T (=KCTC 23999T =KEMB 9004–162T =JCM 18492T).

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Authors and Affiliations

  1. Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 139-774, Republic of Korea

    Jae-** Lee, Eun Sun Joo, Myung Kyum Kim & Sathiyaraj Srinivasan

  2. Microorganism Resources Division, National Institute of Biological Resources, Incheon, 404-170, Republic of Korea

    Myung-Suk Kang

  3. Department of Biotechnology, Hankyong National Univeristy, Kyonggi-do, 456-749, Republic of Korea

    Wan-Taek Im

  4. College of Agricultural and Life Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Hee-Young Jung

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  1. Jae-** Lee
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Correspondence to Sathiyaraj Srinivasan.

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The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DY10T (=KCTC 23999T =KEMB 9004–162T =JCM 18492T) is JQ958375.

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Lee, JJ., Kang, MS., Joo, E.S. et al. Spirosoma montaniterrae sp. nov., an ultraviolet and gamma radiation-resistant bacterium isolated from mountain soil. J Microbiol. 53, 429–434 (2015). https://doi.org/10.1007/s12275-015-5008-5

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