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Enhancing osteogenic bioactivities of coaxial electrospinning nano-scaffolds through incorporating iron oxide nanoparticles and icaritin for bone regeneration

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Abstract

Bone tissue engineering provides a promising strategy for the treatment of bone defects. Nonetheless, the clinical utilization of biomaterial-based scaffolds is constrained by their inadequate mechanical strength and absence of osteo-inductive properties. Here, we proposed to endow nano-scaffold (NS) constructed by coaxial electrospinning technique with enhanced osteogenic bioactivities and mechanical properties by incorporating biocompatible magnetic iron oxide nanoparticles (IONPs) and icaritin (ICA). Four types of nano-scaffolds (NS, ICA@NS, NS-IONPs and ICA@NS-IONPs) were prepared. The incorporation of ICA and IONPs minimally impact their surface morphological and chemical properties. IONPs enhanced the mechanical properties of NS scaffolds, including hardness, tensile strength, and elastic modulus. In vitro assessments demonstrated that ICA@NS-IONPs exhibited enhanced osteogenic bioactivities towards mouse calvarial pre-osteoblast cell line MC3T3-E1 as evidenced by detecting the alkaline phosphatase (ALP) activity level, expressions of osteogenesis-related genes and proteins as well as mineralized nodule formation. Mechanistic investigations revealed that MEK/ERK (MAP kinase-ERK kinase (MEK)/extracellular-signal-regulated kinase (ERK)) signaling pathway could offer a plausible explanation for the osteogenic differentiation of MC3T3-E1 cells induced by ICA@NS-IONPs. Furthermore, the implantation of nano-scaffolds in rat skull defects exhibited a substantial improvement in in vivo bone regeneration. Therefore, IONPs and ICA incorporated coaxial electrospinning nano-scaffolds present a novel strategy for the optimization of scaffolds for bone tissue engineering.

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Acknowledgements

This study was supported by the National Basic Research Program of China (Nos. 2021YFA1201404, and 2019YFA0210103), the National Natural Science Foundation of China (Nos. 32271413, and 82272492), Natural Science Foundation of Jiangsu Province (No. BK20232023), Science program of Jiangsu Province Administration for Market Regulation (No. KJ2024010). The schematic diagram (Scheme 1) was drawn by Figdraw.

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  1. Peng Wang, Qian** Wang, and Dengxian Wu contributed equally to this work.

Authors and Affiliations

  1. School of Biomedical Engineering and Informatics, Nan**g Medical University, Nan**g, 211166, China

    Peng Wang, Shixiong Kang & Hao Wu

  2. State Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nan**g Drum Tower Hospital, The Affiliated Hospital of Nan**g University Medical School, Nan**g, 210008, China

    Peng Wang, Qian** Wang, Dengxian Wu, Zhihong Xu & Qing Jiang

  3. Center of Modem Analysis, Nan**g University, Nan**g, 210008, China

    Yunyang Zhang

  4. State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nan**g, 210009, China

    Peng Wang

  5. Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nan**g Forestry University, Nan**g, 210037, China

    Xucai Wang

  6. Jiangsu Institute of Metrology, Nan**g, 210023, China

    Jiayu Gu

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  1. Peng Wang
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Correspondence to Hao Wu, Zhihong Xu or Qing Jiang.

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Enhancing osteogenic bioactivities of coaxial electrospinning nano-scaffolds through incorporating iron oxide nanoparticles and icaritin for bone regeneration

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Wang, P., Wang, Q., Wu, D. et al. Enhancing osteogenic bioactivities of coaxial electrospinning nano-scaffolds through incorporating iron oxide nanoparticles and icaritin for bone regeneration. Nano Res. (2024). https://doi.org/10.1007/s12274-024-6656-8

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