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ASIC2 Synergizes with TRPV1 in the Mechano-Electrical Transduction of Arterial Baroreceptors

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Abstract

Mechanosensitive ion channels (MSCs) are key molecules in the mechano-electrical transduction of arterial baroreceptors. Among them, acid-sensing ion channel 2 (ASIC2) and transient receptor potential vanilloid subfamily member 1 (TRPV1) have been studied extensively and documented to play important roles. In this study, experiments using aortic arch–aortic nerve preparations isolated from rats revealed that both ASIC2 and TRPV1 are functionally necessary, as blocking either abrogated nearly all pressure-dependent neural discharge. However, whether ASIC2 and TRPV1 work in coordination remained unclear. So we carried out cell-attached patch-clamp recordings in HEK293T cells co-expressing ASIC2 and TRPV1 and found that inhibition of ASIC2 completely blocked stretch-activated currents while inhibition of TRPV1 only partially blocked these currents. Immunofluorescence staining of aortic arch–aortic adventitia from rats showed that ASIC2 and TRPV1 are co-localized in the aortic nerve endings, and co-immunoprecipitation assays confirmed that the two proteins form a compact complex in HEK293T cells and in baroreceptors. Moreover, protein modeling analysis, exogenous co-immunoprecipitation assays, and biotin pull-down assays indicated that ASIC2 and TRPV1 interact directly. In summary, our research suggests that ASIC2 and TRPV1 form a compact complex and function synergistically in the mechano-electrical transduction of arterial baroreceptors. The model of synergism between MSCs may have important biological significance beyond ASIC2 and TRPV1.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31871147 and 31371162) and the Science and Technology Development Program of Bei**g Municipal Education Commission (KZ202010025038).

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Author notes

  1. **aodong Yan and Sitao Zhang have contributed equally to this work.

Authors and Affiliations

  1. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Bei**g, 100069, China

    **aodong Yan, ** Liu, Haixia Huang, Weizhen Niu & Wei Wang

  2. Department of Orthopedics, Xuanwu Hospital, Capital Medical University, Bei**g, 100053, China

    Sitao Zhang

  3. Yan**g Medical College, Capital Medical University, Bei**g, 101300, China

    Haiyan Zhao

  4. Bei**g Laboratory for Cardiovascular Precision Medicine, Capital Medical University, Bei**g, 100069, China

    Wei Wang

  5. Department of Neurobiology, School of Basic Medical Sciences, Bei**g Key Laboratory of Neural Regeneration and Repair, Advanced Innovation Center for Human Brain Protection, Capital Medical University, Bei**g, 100069, China

    Chen Zhang

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  1. **aodong Yan
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  2. Sitao Zhang
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  7. Wei Wang
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Correspondence to Wei Wang or Chen Zhang.

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Yan, X., Zhang, S., Zhao, H. et al. ASIC2 Synergizes with TRPV1 in the Mechano-Electrical Transduction of Arterial Baroreceptors. Neurosci. Bull. 37, 1381–1396 (2021). https://doi.org/10.1007/s12264-021-00737-1

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  • DOI: https://doi.org/10.1007/s12264-021-00737-1

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