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MicroRNA-758 Regulates Oral Squamous Cell Carcinoma via COX-2

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Abstract

The present study is to examine the expression of microRNA (miRNA or miR)-758 in tumor tissues, blood, and serum from patients with oral squamous cell carcinoma (OSCC) and try to investigate its regulatory mechanism on OSCC. Twenty-eight OSCC patients and 36 healthy subjects were included in the study. Tumor tissues and tumor-adjacent tissues were collected from OSCC patients. Peripheral blood and saliva were collected from OSCC patients and healthy subjects. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expression of mRNA and microRNA. Western blotting was carried out to measure protein expression in tissues, while ELISA was employed to determine protein content in serum and saliva. Dual luciferase reporter assay was used to identify the direct interaction between COX-2 mRNA and miR-758. COX-2 mRNA and protein expression was elevated in OSCC patients. In addition, expression of miR-758 was decreased in OSCC patients. miR-758 was able to bind with the 3′-UTR seed region of COX-2 mRNA to regulate its expression. The present study demonstrates that increased expression of COX-2 in tumors, serum, and saliva from OSCC patients is correlated with the decrease of miR-758 expression. miR-758 might regulate OSCC via COX-2 and can be used as a potential gene marker for OSCC.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgments

The authors wish to thank Professor Wei Shang of Qingdao University Medical College and Professor Luqing Wang of Qilu Medical University.

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Authors and Affiliations

  1. Department of Stomatology, Qilu Medical University, Zibo, People’s Republic of China

    Gang Dong, Yan Shi & Chunrong Jiang

  2. Department of Surgical Obstetrics and Gynecology, School of Clinical Medicine, Qilu Medical University, Zibo, People’s Republic of China

    Hong Chen

  3. Weifang Nursing Vocational College, No. 9966, Yunmenshan South Road, Weifang, 262500, Shandong, People’s Republic of China

    Hongtao Yang

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  1. Gang Dong
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  2. Hong Chen
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  3. Yan Shi
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Contributions

GD and HY contributed to the design of the study. GD, HC, YS, and CJ performed the experiments. GD and HY analyzed the data. GD and HY interpreted results and prepared the manuscript. The final version of the manuscript has been read and approved by all authors.

Corresponding author

Correspondence to Hongtao Yang.

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The authors declare that they have no competing interests.

Ethical Approval and Consent to Participate

All procedures performed in the current study were approved by the Ethics Committee of Weifang Nursing Vocational College. Written informed consent was obtained from all patients or their families.

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Written informed consents for publication of any associated data and accompanying images were obtained from all patients or their parents, guardians, or next of kin.

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Dong, G., Chen, H., Shi, Y. et al. MicroRNA-758 Regulates Oral Squamous Cell Carcinoma via COX-2. Indian J Surg 83, 932–938 (2021). https://doi.org/10.1007/s12262-020-02543-x

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  • DOI: https://doi.org/10.1007/s12262-020-02543-x

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