Abstract
The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5′ non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS.
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Alonso S, Izeta A, Sola I, et al. 2002. Transcription regulatory sequences and mRNA expression levels in the coronavirus transmissible gastroenteritis virus. J Virol, 76: 1293–1308.
Ander I, Cristian S, Sara A, et al. 1999. Replication and packaging of transmissible gastroenteritis coronavirusderived synthetic minigenomes. J Virol, 73: 1535–1545.
Ballesteros M L, Sanchez C M, Enjuanes L. 1997. Two Amino Acid Changes at the N-Terminus of Transmissible Gastroenteritis Coronavirus Spike Protein Result in the Loss of Enteric Tropism. Virology, 227: 378–388.
Bredenbeek P J, Pachuk C J, Noten A F, et al. 1990. The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism. Nucl Acids Res, 18: 1825–1830.
Correa I, Jimenez G, Sune C, et al. 1988. Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus. Virus Res, 10: 77–93.
Delmas B, Rasschaert D, Godet M, et al. 1990. Four major antigenic sites of the coronavirus transmissible gastroenteritis virus are located on the amino-terminal half of spike glycoprotein S. J Gen Virol, 71: 1313–1323.
Eleouet J F, Rasschaert D, Lambert P, et al. 1995. Complete Sequence (20 Kilobases) of the Polyprotein-Encoding Gene 1 of Transmissible Gastroenteritis Virus. Virology, 206: 817–822.
Godet M, Grosclaude J, Delmas B, et al. 1994. Major receptor-binding and neutralization determinants are located within the same domain of the transmissible gastroenteritis virus (coronavirus) spike protein. J Virol, 68: 8008–8016.
Isabel C, Fatima G, Maria J B, et al. 1990. Localization of antigenic sites of the E2 glycoprotein of transmissible gastroenteritis coronavirus. J Gen Virol, 71: 271–279.
Kemeny L J, Woods R D. 1977. Quantitative transmissible gastroenteritis virus shedding patterns in lactating sows. Am J Vet Res, 38: 307–310.
Kocherhans R, Bridgen A, Ackermann M, et al. 2001. Completion of the Porcine Epidemic Diarrhoea Coronavirus (PEDV) Genome Sequence. Virus Genes, 23: 137–144.
Woods R D, Wesley R D. 1986. Immune response in sows given transmissible gastroenteritis virus or canine coronavirus. Am J Vet Res, 47: 1239–1242.
Ortego J, Sola I, Almazan F, et al. 2003. Transmissible gastroenteritis coronavirus gene 7 is not essential but influences in vivo virus replication and virulence. Virology, 308: 13–22.
Saif L J, Sestak K. 2006. Transmissible gastroenteritis virus and porcine respiratory coronavirus, In:, Diseases of Swine(Zimmerman, J J, et al. Ed), 9th ed. Iowa: Iowa State University Press, p489–516.
Sanchez C M, Izeta A, Sanches-morgado J M, et al. 1999. Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence. J Virol, 73: 7607–7618.
Serge B, Hubert L. 1995. Site-specific alteration of transmissible gastroenteritis virus spike protein results in markedly reduced pathogenicity. J Gen Virol, 76: 2235–2241.
Siddell S, Wege H, Meulen Ter V. 1983. The Biology of Coronaviruses. J Gen Virol, 64: 761–776.
Simkins R A, Sail L J, Weilnau P A. 1989. Epitope map** and the detection of transmissible gastroenteritis viral proteins in cell culture using biotinylated monoclonal antibodies in a fixed-cell ELISA. Arch Virol, 107: 179–190.
Vaughn E M, Halbur P G, Paul P S. 1995. Sequence comparison of porcine respiratory coronavirus isolates reveals heterogeneity in the S, 3, and 3-1 genes. J Virol, 69: 3176–3184.
Wang Y, Zhang X. 1999. The Nucleocapsid Protein of Coronavirus Mouse Hepatitis Virus Interacts with the Cellular Heterogeneous Nuclear Ribonucleoprotein A1 in Vitro and in Vivo. Virology, 265: 96.
Woods R D. 1976. Leukocyte-aggregation assay for transmissible gastroenteritis of swine. Am J Vet Res, 37: 1405–1408.
Woods R D, Wesley R D. 1986. Immune response in sows given transmissible gastroenteritis virus or canine coronavirus. Am J Vet Res, 47: 1239–1242.
Yang H T, **e W Q, Xue X Y, et al. 2005. Design of wide-spectrum inhibitors targeting coronavirus main proteases. Plos Biology, 10: 1742–1752.
Yin J C, Ren X F, Li Y. 2005. Molecular Cloning and Phylogenetic Analysis of ORF7 Region of Chinese Isolate TH-98 from Transmissible Gastroenteritis Virus. Virus Genes, 30: 395–401.
Zhang X S, Hasoksuz M, Spiro D, et al. 2007. Complete genomic sequences, a key residue in the spike protein and deletions in nonstructural protein 3b of US strains of the virulent and attenuated coronaviruses, transmissible gastroenteritis virus and porcine respiratory coronavirus. Virology, 358: 424–435.
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Foundation items: National Basic Research Program (2004CCA00500); National High-tech Development Research Program of China (2006AA02Z440).
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Li, Jq., Cheng, J., Lan, X. et al. Complete genomic sequence of transmissible gastroenteritis virus TS and 3′ end sequence characterization following cell culture. Virol. Sin. 25, 213–224 (2010). https://doi.org/10.1007/s12250-010-3108-2
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DOI: https://doi.org/10.1007/s12250-010-3108-2