Abstract
Heat shock proteins (HSP) are molecular chaperones involved in many normal cellular processes and environmental stresses. At the genome-wide level, there were no reports on the diversity and phylogeny of the heat shock protein family in Procecidochares utilis. In this study, 43 HSPs were identified from the genome of P. utilis, including 12 small heat shock proteins (sHSPs), 23 heat shock protein 40 (DNAJs), 6 heat shock protein 70 (HSP70s), and 2 heat shock protein 90 (HSP90s). The characteristics of these candidates HSP genes were analyzed by BLAST, and then phylogenetic analysis was carried out. Quantitative real-time PCR (qRT-PCR) was used to analyze the spatiotemporal expression patterns of sHSPs and HSP70s in P. utilis after temperature stress. Results showed that most sHSPs could be induced under heat stress during the adult stage of P. utilis, while a few HSP70s could be induced at the larval stage. This study provides an information framework for the HSP family of P. utilis. Moreover, it lays an important foundation for a better understanding of the role of HSP in the adaptability of P. utilis to various environments.
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Data availability
All supporting data are included within the article and its additional files. The raw reads of the genome and the transcriptomes in this study have been stored in the NCBI database under the accession number PRJNA898965 (genome), SRR11300841 (egg-1), SRR11300840 (egg-2), SRR11300829 (egg-3), SRR11300827 (first instar larvae-1), SRR11300826 (first instar larvae-2), SRR11300825 (first instar larvae-3), SRR11300824 (second instar larvae-1), SRR11300823 (second instar larvae-2), SRR11300822 (second instar larvae-3), SRR11300821 (third instar larvae-1), SRR11300839 (third instar larvae-2), SRR11300838 (third instar larvae-3), SRR11300837 (pupae-1), SRR11300836 (pupae-2), SRR11300835 (pupae-3), SRR11300834 (female-1), SRR11300833 (female-2), SRR11300832 (female-3), SRR11300831 (male-1), SRR11300830 (male-2), and SRR11300828 (male-3).
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Acknowledgements
We thank students Wei Zhao, Mingchuan He, and Yongming Tian (Yunnan Agricultural University) for help collecting insects.
Funding
This work was supported by the National Natural Science Foundation of China (No. 32060640 and 32060639), Reserve Talents Project for Yunnan Young and Middle-aged Academic and Technical Leaders (No. 202205AC160077 and 202105AC160037), and the 15th Science and Technology Innovation and Entrepreneurship Action Fund of Yunnan Agricultural University (No. 2022ZKY581).
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Supplementary information
ESM 1
S1 Table Sequences used for phylogenetic analysis in this work.
ESM 2
S2 Table Primers used in this work.
ESM 3
S3 Table Sequences for the small heat shock proteins identified in the Procecidochares utilis (XLSX).
ESM 4
S4 Table Sequences for the heat shock protein 40 (DNAJ) identified in the Procecidochares utilis (XLSX).
ESM 5
S5 Table Sequences for the heat shock protein 70 and heat shock protein 90 identified in the Procecidochares utilis (XLSX).
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Liang, C., Li, L., Zhao, H. et al. Identification and expression analysis of heat shock protein family genes of gall fly (Procecidochares utilis) under temperature stress. Cell Stress and Chaperones 28, 303–320 (2023). https://doi.org/10.1007/s12192-023-01338-9
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DOI: https://doi.org/10.1007/s12192-023-01338-9