Abstract
The aim of this work was to establish a cost-effective extraction procedure for simultaneous determination of pesticides and veterinary drugs in Chinese market preference fresh aquatic products based on MRLs in food of animal origin in China. Modified QuEChERS method coupled to UPLC-MS/MS for the simultaneous detection of 39 pesticides and veterinary drugs were developed. Six representative matrices of bulk fresh aquatic products in China’s coastal markets showed applicability with recoveries 60.3–119.0% and acceptable precision 4.3–20.8%. Experiments showed that for fresh aquatic product matrices, the addition of EDTA-Mcllvaine effectively improved the extraction efficiency, and 0.4-g C18/0.4 g PSA/1.2-g MgSO4 combination with n-hexane extraction three times can remove the matrix interference effectively. Furthermore, LOD and LOQ in the range of 1.0–5.0 μg/kg and 3.0–10.0 μg/kg for 39 pesticides and veterinary drugs upon six representative matrices of bulk fresh aquatic products, respectively, that providing a practical flexible screening method for production quality control before entering the market.
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Data Availability
The datasets generated during the current study areavailable from the corresponding author upon reasonable request.
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Acknowledgements
This work was supported by Liaoning Provincial Department of Education General Project (LJKMZ20221094), Dalian High Level Talent Innovation Support Program Project (2018RQ78), the Doctor Start of Dalian Ocean University (HDYJ202102), the Scientific Research Youth Project of the Education Department of Liaoning Province (LJKQZ2021122), the National Quality Inspection Public Welfare Industry Scientific Research Special Project (201410059), **ngliao Talent Plan Agricultural Experts, Sub project of Agricultural Science and Technology Talents (XLYC2213043). The authors wish to thank the management of the above departments for their support of this study.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Liu Yijun, Jiang Dan, Liu Mengyao, Ding Jian, Liu Yang and Hu **a. The first draft of the manuscript was written by Li Shuo and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Li, S., Liu, Y., Jiang, D. et al. Simultaneous Quantification of 39 Pesticides and Veterinary Drug Residues in Aquaculture Products Using Ultra Performance Liquid Chromatography Tandem Mass Spectrometry with Modified QuEChERS. Food Anal. Methods 17, 797–811 (2024). https://doi.org/10.1007/s12161-024-02602-z
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DOI: https://doi.org/10.1007/s12161-024-02602-z