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An Electrochemical Immunoassay for Lactobacillus rhamnosus GG Using Cu@Cu2O Nanoparticle-Embedded B, N, Co-doped Porous Carbon

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Abstract

In this paper, we have developed a fast and low-cost label-free electrochemical immunosensor for detection of Lactobacillus rhamnosus GG (LGG). The proposed sensor is based on a glassy carbon electrode modified with Cu@Cu2O nanoparticles anchored B, N co-doped porous carbon (BNPC) composite. The copper-derived nanoparticles with multiple valence states undergo dramatic changes in surface areas, conductivity, and actives sites due to their combination with BNPC, resulting in enhanced electrochemical performance. In the amperometric immunoassay, the immune complex of antigen and antibody inhibits the transfer of electrons on the electrode interface, causing a change in the current. Thus the quantitative detection of LGG is achieved. Differential pulse voltammetry (DPV) was performed at a LGG concentration of 102 to 108 colony-forming units CFU mL−1. The immunosensor showed a good linear relationship between the decrease in peak current and the concentration of LGG (R2 = 0.9934), with a short detection time and a detection limit of 12 CFU mL−1. In short, we have developed a highly sensitive electrochemical immunosensor for the specific detection of LGG using the current-mode, label-free immunoassay method evaluated in this study, providing a reliable mean for quantifying probiotics.

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Funding

Funding was received from the following: Natural Science Foundation (No. 32001743 and 32001661), Natural science fund for colleges and universities in Jiangsu Province (No. 19KJB550002), State Key Laboratory of Analytical Chemistry for Life Science (No. SKLACLS2001), Nan**g University.

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Authors and Affiliations

  1. School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225127, PR China

    Jiamin Wang, Wenyuan Zhou, Lu Gao, Zhenquan Yang, Dawei Chen & Yajun Gao

  2. Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou, Jiangsu, 225127, PR China

    Zhenquan Yang, Dawei Chen & Yajun Gao

  3. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu, 225002, P. R. China

    Zhanjun Yang

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  1. Jiamin Wang
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  2. Wenyuan Zhou
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  3. Lu Gao
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Contributions

Jiamin Wang: investigation, writing-original draft preparation. Wenyuan Zhou: experimental work. Lu Gao: reviewing and Editing. Zhenquan Yang: conceptualization, Reviewing. Zhanjun Yang: Conceptualization. Dawei Chen: data curation, reviewing and editing. Yajun Gao: conceptualization, reviewing, and editing.

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Correspondence to Zhanjun Yang or Yajun Gao.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Conflict of Interest

Author Jiamin Wang declares that he has no conflict of interest. Author Wenyuan Zhou declares that he has no conflict of interest. Author Lu Gao declares that she has no conflict of interest. Author Zhenquan Yang declares that he has no conflict of interest. Author Zhanjun Yang declares that she has no conflict of interest. Author Dawei Chen declares that he has no conflict of interest. Author Yajun Gao declares that he has no conflict of interest.

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Wang, J., Zhou, W., Gao, L. et al. An Electrochemical Immunoassay for Lactobacillus rhamnosus GG Using Cu@Cu2O Nanoparticle-Embedded B, N, Co-doped Porous Carbon. Food Anal. Methods 15, 3379–3389 (2022). https://doi.org/10.1007/s12161-022-02373-5

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