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Magneto Immunofluorescence Assay for Quinolone Detection in Bovine Milk

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A Correction to this article was published on 30 May 2020

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Abstract

Quinolone is a family of widely prescript antibiotics in veterinary medicine. Their excessive use can be causative of quinolone residues in foodstuffs of animal origin. Therefore, a magneto immunofluorescence assay in microwell plate has been developed and validated to screen quinolone in bovine milk. The assay is based on an indirect competitive method where the quinolone in the milk sample competed with the quinolone immobilized on magnetic beads for the anti-quinolone antibody. Then, an anti-IgG antibody–labeled peroxidase and their respective substrates were added and the fluorescent signal was indirectly related with the quinolone concentration. Recoveries of quinolones from 82 to 102% were obtained in skimmed, whole, and raw milk samples in concordance with the results obtained by a reference method. Seven quinolones were evaluated quantitatively by the method, obtaining limits of detection of 10 μg L−1 for ciprofloxacin and marbofloxacin, 13 μg L−1 for enrofloxacin and danofloxacin, 22 μg L−1 for norfloxacin, 29 μg L−1 for sarafloxacin, and 30 μg L−1 for ofloxacin. For the screening detection of quinolones, ciprofloxacin was used as model with decision limit (CCα) and detection capability (CCβ) of 103 and 106 μg L−1, respectively. High cross-reactivity (> 100%) was obtained to all assayed quinolones while negligible cross-reactivity (< 0.1%) to other antibiotics (β-lactams, aminoglycosides, macrolides, tetracyclines, and sulfamides) was observed. The validation provided evidence that the method is suitable to be applied in routine analysis for the detection of quinolones in bovine milk.

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Change history

  • 30 May 2020

    The article Magneto Immunofluorescence Assay for Quinolone Detection in Bovine Milk, written by Silvina V. Kergaravat, Orlando G. Nagel, Rafael L. Althaus and Silvia R. Hernández was originally published electronically on the publisher’s internet portal (currently SpringerLink) on 30 March 2020 with open access. With the author(s)’ decision to step back from Open Choice, the copyright of the article changed on May 2020 to © Springer Science+Business Media, LLC, part of Springer Nature 2020 and the article is forthwith distributed under the terms of copyright.

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Funding

This work was supported by the Agencia Santafesina de Ciencia, Tecnología e Innovación (ASaCTeI 2016 (2010-092-16)); by Universidad Nacional del Litoral (CAI+D 2016 (PI 50020150100068LI), CAI+D 2016 (PI 50120150100130LI), CAI+D orientado 2016/III Sistema Productivo Cód); and by Agencia Nacional de Promoción Científica y Tecnológica: PICT 2017 (Cod. 2841).

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Authors and Affiliations

  1. Laboratorio de Sensores y Biosensores, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000, Santa Fe, Argentina

    Silvina V. Kergaravat & Silvia R. Hernández

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CCT Santa Fe, 3000, Santa Fe, Argentina

    Silvina V. Kergaravat

  3. Cátedra de Biofísica, Departamento de Ciencias Básicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 3080, Esperanza, Argentina

    Orlando G. Nagel & Rafael L. Althaus

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  1. Silvina V. Kergaravat
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  2. Orlando G. Nagel
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  3. Rafael L. Althaus
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  4. Silvia R. Hernández
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Correspondence to Silvina V. Kergaravat.

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Silvina V. Kergaravat declares that she has no conflict of interest. Orlando G. Nagel declares that he has no conflict of interest. Rafael L. Althaus declares that he has no conflict of interest. Silvia R. Hernández declares that she has no conflict of interest.

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Kergaravat, S.V., Nagel, O.G., Althaus, R.L. et al. Magneto Immunofluorescence Assay for Quinolone Detection in Bovine Milk. Food Anal. Methods 13, 1539–1547 (2020). https://doi.org/10.1007/s12161-020-01749-9

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