Log in

Mid-Infrared Spectroscopy (MIR) for Simultaneous Determination of Fat and Protein Content in Meat of Several Animal Species

  • Published:
Food Analytical Methods Aims and scope Submit manuscript

Abstract

The aim of this research was to study the application of MIR spectroscopy as an alternative to conventional methods to determine fat and protein content. Samples of the main species used to produce meat products were analyzed, showing all of them absorption bands at similar wavenumbers though with different intensity. Correlation analysis of absorption intensities showed that bands around 2925, 2854, and 1746 cm−1 are associated with fat content, whereas bands around 3288, 1657, and 1542 cm−1 are associated with proteins. During the validation process, prediction models of fat and protein content were successfully obtained with R 2 0.9173 and 0.7534, respectively. Finally, a good result (R 2 = 0.8829) was obtained on the estimation of the lipid content when the information at only one wavenumber was used.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Alamprese C, Casale M, Sinelli N, Lanteri S, Casiraghi E (2013) Detection of minced beef adulteration with turkey meat by UV-vis, NIR and MIR spectroscopy. Lwt-Food Sci Technol 53:225–232

    Article  CAS  Google Scholar 

  • Al-Jowder O, Kemsley EK, Wilson RH (1997) Mid-infrared spectroscopy and authenticity problems in selected meats: a feasibility study. Food Chem 59:195–201

    Article  CAS  Google Scholar 

  • Al-Jowder O, Defernez M, Kemsley EK, Wilson RH (1999) Mid-infrared spectroscopy and chemometrics for the authentication of meat products. J Agric Food Chem 47:3210–3218

    Article  CAS  Google Scholar 

  • Al-Jowder O, Kemsley EK, Wilson RH (2002) Detection of adulteration in cooked meat products by mid-infrared spectroscopy. J Agric Food Chem 50:1325–1329

    Article  CAS  Google Scholar 

  • Arce L (2009) Feasibility study on the use of infrared spectroscopy for the direct authentication of Iberian pig fattening diet. Anal Chim Acta 636:183–189

    Article  CAS  Google Scholar 

  • Carbonaro M, Nucara A (2010) Secondary structure of food proteins by Fourier transform spectroscopy in the mid-infrared region. Amino Acids 38:679–690

    Article  CAS  Google Scholar 

  • De Smet S, Raes K, Demeyer D (2004) Meat fatty acid composition as affected by fatness and genetic factors: a review. Anim Res 53:81–98

    Article  CAS  Google Scholar 

  • Ellis DI, Broadhurst D, Kell DB, Rowland JJ, Goodacre R (2002) Rapid and quantitative detection of the microbial spoilage of meat by Fourier transform infrared spectroscopy and machine learning. Appl Environ Microbiol 68:2822–2828

    Article  CAS  Google Scholar 

  • Etzion Y, Linker R, Cogan U, Shmulevich I (2004) Determination of protein concentration in raw milk by mid-infrared Fourier transform infrared/attenuated total reflectance spectroscopy. J Dairy Sci 87:2779–2788

    Article  CAS  Google Scholar 

  • Guillen MD (2000) Some of the most significant changes in the Fourier transform infrared spectra of edible oils under oxidative conditions. J Sci Food Agric 80:2028

    Article  CAS  Google Scholar 

  • Hernández-Martínez M (2013) Prediction of total fat, fatty acid composition and nutritional parameters in fish fillets using MID-FTIR spectroscopy and chemometrics. LWT Food Sci Technol 52:12–20

    Article  Google Scholar 

  • ISO 1442:1973 (1973) Meat and meat products—determination of moisture content

  • ISO 1443:1973 (1973) Meat and meat products—determination of total fat content

  • ISO 937:1978 (1978) Meat and meat products—determination of nitrogen content (Reference method)

  • Karoui R, Downey G, Blecker C (2010) Mid-infrared spectroscopy coupled with chemometrics: a tool for the analysis of intact food systems and the exploration of their molecular structure-quality relationships—a review. Chem Rev 110:6144–6168

    Article  CAS  Google Scholar 

  • Meza-Márquez OG (2010) Application of mid-infrared spectroscopy with multivariate analysis and soft independent modeling of class analogies (SIMCA) for the detection of adulterants in minced beef. Meat Sci 86:511–519

    Article  Google Scholar 

  • Meza-Marquez OG, Gallardo-Velazquez T, Osorio-Revilla G, Dorantes-Alvarez L (2012) Detection of clenbuterol in beef meat, liver and kidney by mid-infrared spectroscopy (FT-mid IR) and multivariate analysis. Int J Food Sci Technol 47:2342–2351

    Article  CAS  Google Scholar 

  • Ordoudi SA, de los Mozos Pascual M, Tsimidou MZ (2014) On the quality control of traded saffron by means of transmission Fourier-transform mid-infrared (FT-MIR) spectroscopy and chemometrics. Food Chem 150:414–421

    Article  CAS  Google Scholar 

  • Papadopoulou O (2011) Contribution of Fourier transform infrared (FTIR) spectroscopy data on the quantitative determination of minced pork meat spoilage. Food Res Int 44:3264–3271

    Article  CAS  Google Scholar 

  • Pretsch E, Bühlmann P, Affolter C (2000). Structure determination of organic compounds: tables of spectral data. Springer

  • Rannou H, Downey G (1997) Discrimination of raw pork, chicken and turkey meat by spectroscopy in the visible, near- and mid-infrared ranges. Anal Commun 34:401–404

    Article  CAS  Google Scholar 

  • Rohman A (2011) Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy. Meat Sci 88:91–95

    Article  CAS  Google Scholar 

  • Shiroma C, Rodriguez-Solona L (2009) Application of NIR and MIR spectroscopy in quality control of potato chips. J Food Compos Anal 22:596–605

    Article  CAS  Google Scholar 

  • Skoog DA, Crouch SR, Holler FJ, Anzures MB (2008) Principles of instrumental analysis. Cengage Learning Latin America

  • Sun DW (2009) Infrared spectroscopy for food quality analysis and control. Elsevier Science

  • Wold JP, O’Farrell M, Høy M, Tschudi J (2011) On-line determination and control of fat content in batches of beef trimmings by NIR imaging spectroscopy. Meat Sci 89:317–324

    Article  CAS  Google Scholar 

  • Wu D, Feng SJ, He C, He Y (2008) NIRS and MIRS technique for the determination of protein and fat content in milk powder - art. no. 66251S. International Symposium on Photoelectronic Detection and Imaging 2007. Related Technologies and Applications 6625:S6251–S6251

    Google Scholar 

  • Wu D, He Y, Shi J, Feng S (2009) Exploring near and midinfrared spectroscopy to predict trace iron and zinc contents in powdered milk. J Agric Food Chem 57:1697–1704

    Article  CAS  Google Scholar 

Download references

Acknowledgements

In memoriam of our beloved friend Mario Sorolla Ayza.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. J. Beriain.

Ethics declarations

Funding

This work was supported by the Spanish Government through the Consolider Engineering Metamaterials under Contract CSD2008-00066 and Contract TEC2011-28664-C02. The FTIR spectrometer was financed by the Spanish Ministry of Economy and Competitiveness in the frame of the project CEI10-2-2005. M. Beruete acknowledges funding by the Spanish Government under the research contract program Ramón y Cajal RYC-2011-08221.

Conflict of Interest

M. Lozano declares that she has no conflict of interest. P. Rodríguez-Ulibarri declares that he has no conflict of interest. J. C. Echeverría declares that he has no conflict of interest. M. Beruete declares that he has no conflict of interest. M. Sorolla declares that he has no conflict of interest. M. J. Beriain declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lozano, M., Rodríguez-Ulibarri, P., Echeverría, J.C. et al. Mid-Infrared Spectroscopy (MIR) for Simultaneous Determination of Fat and Protein Content in Meat of Several Animal Species. Food Anal. Methods 10, 3462–3470 (2017). https://doi.org/10.1007/s12161-017-0879-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12161-017-0879-1

Keywords

Navigation