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Determination of Egg Yolk Immunoglobulin by Resonance Light Scattering of Affinity-Labeled Au Nanoparticles

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Abstract

Chicken egg yolk immunoglobulin (IgY) is the only antibody existing in chicken egg yolk, which has many significant advantages. We developed an immunity affinity biosensor system based on resonance Rayleigh scattering (RRS) and using immunity affinity rabbit anti-chicken IgY (anti-IgY) labeling Au nanoparticles (AuNPs). This system was rapid and sensitive for IgY detection due to the IgY-anti-IgY affinitive immunity interaction. The RRS and absorption spectra of AuNPs and the binding system were analyzed. In addition, the effects of several factors on the scattering intensity were investigated. Under optimal conditions, RRS intensity of anti-IgY-AuNPs increase linearly with an increasing concentration of IgY from 4 to 200 ng/mL. The limit of detection was 1.92 ng/mL. This rapid and selective IgY detection method was used in synthetic samples and egg products with recoveries between 89.86 and 110.25 %, thereby demonstrating the feasible and practical application of this assay.

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Authors and Affiliations

  1. National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Qi Wang, Shan Wu, Meihu Ma & Zhaoxia Cai

Authors
  1. Qi Wang
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  2. Shan Wu
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  3. Meihu Ma
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  4. Zhaoxia Cai
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Correspondence to Zhaoxia Cai.

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Funding

This study was funded by the National Natural Science Foundation of China (No. 31371810) and Central University basic scientific research business expenses special funds project (2662015PY080).

Conflict of Interest

Qi Wang declares that she has no conflict of interest. Shan Wu declares that she has no conflict of interest. Meihu Ma declares that he has no conflict of interest. Zhaoxia Cai declares that she has no conflict of interest.

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This article does not contain any studies with human participants performed by any of the authors.

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Wang, Q., Wu, S., Ma, M. et al. Determination of Egg Yolk Immunoglobulin by Resonance Light Scattering of Affinity-Labeled Au Nanoparticles. Food Anal. Methods 9, 2052–2059 (2016). https://doi.org/10.1007/s12161-015-0391-4

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  • DOI: https://doi.org/10.1007/s12161-015-0391-4

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