Abstract
The development and use of renewable energy resources is a crucial solution for a sustainable energy strategy to decrease the dependence on fossil fuels. Lignocellulosic ethanol has gained recognition as a renewable energy resource vital for sustainable development. Currently, the research and industry sectors utilize a single type of lignocellulose biomass for ethanol production. However, this biomass dependency is a potential risk due to the global warming effect on biomass plantations. This study assessed the versatility of rice straw (RS), Napier grass (NG), and sugarcane bagasse (SB) as a mixed biomass for bioethanol production. The mixture of equal proportion of RS, NG, and SB in a 1:1:1 ratio produced higher concentration of bioethanol than individual biomasses. NaOH-pretreated samples were more effective than H2SO4 pretreated and untreated samples in bioethanol production. The NaOH-pretreated mixed sample yielded maximum bioethanol of 0.82% (v/v). About 0.43 g/g and 0.12 g/g of reducing sugars and ethanol, respectively, could be produced using RS, NG, and SB in the ratio of 1:1:1. This research indicates that different biomass types can replace one another in the event of limited resources, thus reducing the dependency on a particular biomass type for biorefinery.
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References
Mukherjee A, Banerjee S, Halder G (2018) Parametric optimization of delignification of rice straw through central composite design approach towards application in grafting. J Adv Res 14:11–23. https://doi.org/10.1016/j.jare.2018.05.004
Saravanan A, Kumar PS, Jeevanantham S, Karishma S, Vo D-VN (2022) Recent advances and sustainable development of biofuels production from lignocellulosic biomass. Bioresour Technol 344:126203. https://doi.org/10.1016/j.biortech.2021.126203
Das PK, Das BP, Dash P (2020) Potentials of postharvest rice crop residues as a source of biofuel. In: Praveen Kumar R, Gnansounou E, Raman JK, Baskar G (eds) Refining biomass residues for sustainable energy and bioproducts. Academic Press, pp 275–301. https://doi.org/10.1016/B978-0-12-818996-2.00013-2
Cotta MA (2012) Ethanol production from lignocellulosic biomass by recombinant Escherichia coli strain FBR5. Bioengineered 3(4):197–202. https://doi.org/10.4161/bioe.19874
Negawo AT, Teshome A, Kumar A, Hanson J, Jones CS (2017) Opportunities for Napier grass (Pennisetum purpureum) improvement using molecular genetics. Agronomy 7(2):28. https://doi.org/10.3390/agronomy7020028
Nimmanterdwong P, Chalermsinsuwan B, Østergård H, Piumsomboon P (2017) Environmental performance assessment of Napier grass for bioenergy production. J Clean Prod 165:645–655. https://doi.org/10.1016/j.jclepro.2017.07.126
Kamarullah SH, Mydin MM, Omar W, Harith SS, Noor BHM, Alias NZA, Manap S, Mohamad R (2015) Surface morphology and chemical composition of Napier grass fibers. Malays J Anal Sci 19(4):889–895
Minmunin J, Limpitipanich P, Promwungkwa A (2015) Delignification of elephant grass for production of cellulosic intermediate. Energy Procedia 79:220–225. https://doi.org/10.1016/j.egypro.2015.11.468
Mensah RQ, Yingkamheang N, Venkatachalam P, Show P-L, Mussatto SI, Sriariyanun M, Sukyai P, Parakulsuksatid P, Rattanaporn K (2023) Application of green produced xylooligosaccharides from sugarcane residues and their properties–recent progress towards sustainability. Bioresour Technol Rep 23:101537. https://doi.org/10.1016/j.biteb.2023.101537
AboDalam H, Devra V, Ahmed FK, Li B, Abd-Elsalam KA (2022) Rice wastes for green production and sustainable nanomaterials: an overview. In: Abd-Elsalam KA, Periakaruppan R, Rajeshkumar S (eds) Nanobiotechnology for Plant Protection,Agri-Waste and Microbes for Production of Sustainable Nanomaterials. Elsevier, pp 707–728. https://doi.org/10.1016/B978-0-12-823575-1.00009-3
Pinzi S, Dorado M (2011) Vegetable-based feedstocks for biofuels production. Handbook of biofuels production. In: Luque R, Campelo J, Clark J (eds) Woodhead Publishing Series in Energy, Handbook of Biofuels Production. Woodhead Publishing, pp 61–94. https://doi.org/10.1533/9780857090492.1.61
Sriariyanun M, Gundupalli MP, Phakeenuya V, Phusamtisampan T, Cheng Y-S, Venkatachalam P (2023) Biorefinery approaches for production of cellulosic ethanol fuel using recombinant engineered microorganisms. J Appl Sci Eng 27:1985–2005. https://doi.org/10.6180/jase.202402_27(2).0001
Chen H, Liu J, Chang X, Chen D, Xue Y, Liu P, Lin H, Han S (2017) A review on the pretreatment of lignocellulose for high-value chemicals. Fuel Process Technol 160:196–206. https://doi.org/10.1016/j.fuproc.2016.12.007
Chundawat SP, Beckham GT, Himmel ME, Dale BE (2011) Deconstruction of lignocellulosic biomass to fuels and chemicals. Annu Rev Chem Biomol Eng 2:121–145
Panakkal EJ, Cheenkachorn K, Gundupalli MP, Kitiborwornkul N, Sriariyanun M (2021) Impact of sulfuric acid pretreatment of durian peel on the production of fermentable sugar and ethanol. J Indian Chem Soc 98(12):100264. https://doi.org/10.1016/j.jics.2021.100264
Gundupalli MP, Kajiura H, Ishimizu T, Bhattacharyya D (2020) Alkaline hydrolysis of coconut pith: process optimization, enzymatic saccharification, and nitrobenzene oxidation of Kraft lignin. Biomass Convers Biorefin 1–19. https://doi.org/10.1007/s13399-020-00890-z
Madadi M, Wang Y, Zhang R, Hu Z, Gao H, Zhan D, Yu H, Yang Q, Wang Y, Tu Y (2022) Integrating mild chemical pretreatments with endogenous protein supplement for complete biomass saccharification to maximize bioethanol production by enhancing cellulases adsorption in novel bioenergy Amaranthus. Ind Crops Prod 177:114471. https://doi.org/10.1016/j.indcrop.2021.114471
Dharmalingam B, Tantayotai P, Panakkal EJ, Cheenkachorn K, Kirdponpattara S, Gundupalli MP, Cheng Y-S, Sriariyanun M (2022) Organic acid pretreatments and optimization techniques for mixed vegetable waste biomass conversion into biofuel production. BioEnergy Res 1–16. https://doi.org/10.1007/s12155-022-10517-y
Trilokesh C, Uppuluri KB (2021) Biobutanol from lignocellulosic biomass and microalgae: Scope, technology, and economics. In: Ray RC (ed) Applied Biotechnology Reviews, Sustainable Biofuels. Academic Press, pp 163–223. https://doi.org/10.1016/B978-0-12-820297-5.00008-6
Kumar R, Strezov V, Weldekidan H, He J, Singh S, Kan T, Dastjerdi B (2020) Lignocellulose biomass pyrolysis for bio-oil production: a review of biomass pre-treatment methods for production of drop-in fuels. Renew Sustain Energy Rev 123:109763. https://doi.org/10.1016/j.rser.2020.109763
Gonzales RR, Kumar G, Sivagurunathan P, Kim S-H (2017) Enhancement of hydrogen production by optimization of pH adjustment and separation conditions following dilute acid pretreatment of lignocellulosic biomass. Int J Hydrog Energy 42(45):27502–27511. https://doi.org/10.1016/j.ijhydene.2017.05.021
Hashemi B, Sarker S, Lamb JJ, Lien KM (2021) Yield improvements in anaerobic digestion of lignocellulosic feedstocks. J Clean Prod 288:125447. https://doi.org/10.1016/j.jclepro.2020.125447
Balan V (2014) Current challenges in commercially producing biofuels from lignocellulosic biomass. ISRN Biotechnol 2014:463074. https://doi.org/10.1155/2014/463074
Saïed N, Khelifi M, Bertrand A, Tremblay GF, Aider M (2023) Effects of acid and alkali pretreatments on carbohydrate release from sweet sorghum and sweet pearl millet bagasse for bioethanol production. BioEnergy Res 1–15. https://doi.org/10.1007/s12155-023-10617-3
Panakkal EJ, Sriariyanun M, Ratanapoompinyo J, Yasurin P, Cheenkachorn K, Rodiahwati W, Tantayotai P (2022) Influence of sulfuric acid pretreatment and inhibitor of sugarcane bagasse on the production of fermentable sugar and ethanol. Appl Sci Eng Progress 15(1). https://doi.org/10.1016/B978-0-12-820297-5.00008-6
Tantayotai P, Gundupalli MP, Katam K, Rattanaporn K, Cheenkachorn K, Sriariyanun M (2022) In-depth investigation of the bioethanol and biogas production from organic and mineral acid pretreated sugarcane bagasse: comparative and optimization studies. Biocatal Agric Biotechnol 45:102499. https://doi.org/10.1016/j.bcab.2022.102499
Yamamoto Y, Kishimura H, Kinoshita Y, Saburi W, Kumagai Y, Yasui H, Ojima T (2019) Enzymatic production of xylooligosaccharides from red alga dulse (Palmaria sp.) wasted in Japan. Process Biochem 82:117–122. https://doi.org/10.1016/j.procbio.2019.03.030
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426–428. https://doi.org/10.1021/ac60147a030
Panakkal EJ, Cheenkachorn K, Chuetor S, Tantayotai P, Raina N, Cheng Y-S, Sriariyanun M (2022) Optimization of deep eutectic solvent pretreatment for bioethanol production from Napier grass. Sustain Energy Technol Assess 54:102856. https://doi.org/10.1016/j.seta.2022.102856
Sriariyanun M, Kitiborwornkul N, Tantayotai P, Rattanaporn K, Show P-L (2022) One-pot ionic liquid-mediated bioprocess for pretreatment and enzymatic hydrolysis of rice straw with ionic liquid-tolerance bacterial cellulase. Bioengineering 9(1):17. https://doi.org/10.3390/bioengineering9010017
Sriariyanun M, Mutrakulcharoen P, TepaamornDech S, Cheenkachorn K, Rattanaporn K (2019) A rapid spectrophotometric method for quantitative determination of ethanol in fermentation products. Orient J Chem 35(2):744–750. https://doi.org/10.13005/ojc/350234
Bakker R, Elbersen H, Poppens R, Lesschen JP (2013) Rice straw and wheat straw-potential feedstocks for the biobased economy. NL Agency Ministry of Economic Affairs, Wageningen UR, Food & Biobased Research, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands
Gundupalli MP, Sahithi SA, Jayex EP, Asavasanti S, Yasurin P, Cheng Y-S, Sriariyanun M (2022) Combined effect of hot water and deep eutectic solvent (DES) pretreatment on a lignocellulosic biomass mixture for improved saccharification efficiency. Bioresour Technol Rep 17:100986. https://doi.org/10.1016/j.biteb.2022.100986
Kulshreshtha A (2022) Sustainable energy generation from municipal solid waste. In: Hussain CM, Singh S, Goswami L (eds) Waste-to-Energy Approaches Towards Zero Waste. Elsevier, pp 315–342. https://doi.org/10.1016/B978-0-323-85387-3.00005-7
Tajmirriahi M, Momayez F, Karimi K (2021) The critical impact of rice straw extractives on biogas and bioethanol production. Bioresour Technol 319:124167. https://doi.org/10.1016/j.biortech.2020.124167
Das SP, Gupta A, Das D, Goyal A (2016) Enhanced bioethanol production from water hyacinth (Eichhornia crassipes) by statistical optimization of fermentation process parameters using Taguchi orthogonal array design. Int Biodeterior Biodegrad 109:174–184. https://doi.org/10.1016/j.ibiod.2016.01.008
Rezania S, Alizadeh H, Park J, Din MFM, Darajeh N, Ebrahimi SS, Saha BB, Kamyab H (2019) Effect of various pretreatment methods on sugar and ethanol production from cellulosic water hyacinth. BioResources 14(1):592–606
Paz-Cedeno FR, Carceller JM, Iborra S, Donato RK, Rodriguez AFR, Morales MA, Solorzano-Chavez EG, Roldán IUM, de Paula AV, Masarin F (2022) Stability of the Cellic CTec2 enzymatic preparation immobilized onto magnetic graphene oxide: assessment of hydrolysis of pretreated sugarcane bagasse. Ind Crops Prod 183:114972. https://doi.org/10.1016/j.indcrop.2022.114972
Elalami D, Barakat A (2021) State of the art of energy production from agricultural residues using thermochemical and biological processes. Clean Energy and Resources Recovery, vol 1. Biomass Waste Based Biorefineries. Elsevier, pp 1–24. https://doi.org/10.1016/B978-0-323-85223-4.00008-7
Sanchis-Sebastiá M, Gomis-Fons J, Galbe M, Wallberg O (2020) Techno-economic evaluation of biorefineries based on low-value feedstocks using the BioSTEAM software: a case study for animal bedding. Processes 8(8):904. https://doi.org/10.3390/pr8080904
Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83(1):1–11. https://doi.org/10.1016/S0960-8524(01)00212-7
Nguyen QA, Yang J, Bae H-J (2017) Bioethanol production from individual and mixed agricultural biomass residues. Ind Crops Prod 95:718–725. https://doi.org/10.1016/j.indcrop.2016.11.040
Kumar N, Yadav A, Singh G, Singh A, Kumar P, Aggarwal NK (2023) Comparative study of ethanol production from sodium hydroxide pretreated rice straw residue using Saccharomyces cerevisiae and Zymomonas mobilis. Arch Microbiol 205(4):146. https://doi.org/10.1007/s00203-023-03468-1
Tsai MH, Lee WC, Kuan WC, Sirisansaneeyakul S, Savarajara A (2018) Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production. Energy Sci Eng 6(6):683–692. https://doi.org/10.1002/ese3.243
de Carvalho DM, de Queiroz JH, Colodette JL (2016) Assessment of alkaline pretreatment for the production of bioethanol from eucalyptus, sugarcane bagasse and sugarcane straw. Ind Crops Prod 94:932–941. https://doi.org/10.1016/j.indcrop.2016.09.069
Kannah RY, Sivashanmugham P, Kavitha S, Banu JR (2020) Valorization of food waste for bioethanol and biobutanol production. In: Rajesh Banu J, Kumar G, Gunasekaran M, Kavitha S (eds) Food Waste to Valuable Resources. Academic Press, pp 39–73. https://doi.org/10.1016/B978-0-12-818353-3.00003-1
Yirgu Z, Leta S, Hussen A, Khan MM (2023) Pretreatment and optimization of reducing sugar extraction from indigenous microalgae grown on brewery wastewater for bioethanol production. Biomass Convers Biorefin 13(8):6831–6845. https://doi.org/10.1007/s13399-021-01779-1
Guo H, Zhao Y, Chang J-S, Lee D-J (2022) Inhibitor formation and detoxification during lignocellulose biorefinery: a review. Bioresour Technol 361:127666. https://doi.org/10.1016/j.biortech.2022.127666
**e X, Chen M, Tong W, Song K, Wang J, Wu S, Hu J, ** Y, Chu Q (2023) Comparative study of acid-and alkali-catalyzed 1, 4-butanediol pretreatment for co-production of fermentable sugars and value-added lignin compounds. Biotechnol Biofuels Bioprod 16(1):1–16. https://doi.org/10.1186/s13068-023-02303-5
Abeysuriya DI, Sethunga G, Rathnayake M (2023) Process simulation–based scenario analysis of scaled-up bioethanol production from water hyacinth. Biomass Convers Biorefin 1–16. https://doi.org/10.1007/s13399-023-03891-w
Todri E, Amenaghawon AN, Del Val IJ, Leak DJ, Kontoravdi C, Kucherenko S, Shah N (2014) Global sensitivity analysis and meta-modeling of an ethanol production process. Chem Eng Sci 114:114–127. https://doi.org/10.1016/j.ces.2014.04.027
Ovalles C, Rogel E, Morazan H, Moir ME (2018) The importance of mass balances: case studies of evaluation of asphaltene dispersants and antifoulants. The Boduszynski continuum: contributions to the understanding of the molecular composition of petroleum. ACS Symposium Series. American Chemical Society, pp 25–49. https://doi.org/10.1021/bk-2018-1282.ch002
Shukla A, Kumar D, Girdhar M, Kumar A, Goyal A, Malik T, Mohan A (2023) Strategies of pretreatment of feedstocks for optimized bioethanol production: distinct and integrated approaches. Biotechnol Biofuels Bioprod 16(1):44. https://doi.org/10.1186/s13068-023-02295-2
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This work was financially supported by National Science, Research and Innovation Fund (NSRF), King Mongkut’s University of Technology North Bangkok (Grant Contract No. KMUTNB-67-KNOW-07) and Srinakharinwirot University.
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KC: investigation, writing—original draft, conceptualization. RQM: methodology, reviewing and editing. BD: investigation, writing—original draft. MPG: reviewing and editing. KR: reviewing and editing, data curation. PT: data curation, funding acquisition. PLS: reviewing and editing. MS: conceptualization, data curation, writing—reviewing and editing, funding acquisition, project administration.
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Cheenkachorn, K., Mensah, R.Q., Dharmalingam, B. et al. The Versatility of Mixed Lignocellulose Feedstocks for Bioethanol Production: an Experimental Study and Empirical Prediction. Bioenerg. Res. 17, 1004–1014 (2024). https://doi.org/10.1007/s12155-023-10705-4
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DOI: https://doi.org/10.1007/s12155-023-10705-4