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Differential Angiogenic Induction Impacts Nasal Polyp Tissue Growth

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Abstract

In chronic rhinosinusitis with nasal polyps, inflammatory edema drives tissue remodeling favoring anomalous growth of the nasal mucosa, but a proangiogenic contribution of nasal polyp in support of tissue growth is still controversial. The chorioallantoic membrane of chicken embryo model was employed to address the potentiality of nasal tissue fragments to modulate angiogenesis. Fifty-seven fertilized eggs were implanted with polyp or healthy nasal mucosa tissue or were kept as non-implanted controls. The embryos’ size, length, and development stage, and chorioallantoic membrane vasculature morphology were evaluated after 48 h. Quantitative computer vision techniques applied to digital chorioallantoic membrane images automatically calculated the branching index as the ratio between the areas of the convex polygon surrounding the vascular tree and the vessels’ area. Ethics approval and consent to participate: the study was approved by the Human Research Ethics Committee of the Federal University of São Paulo (CAAE number: 80763117.1.0000.5505) and by the Animal Research Ethics Committee of University of São Paulo (nº CEUA 602–2019). Mucosal, but not polyp tissue implants, hampered embryo development and induced underdeveloped chorioallantoic membranes with anastomosed, interrupted, and regressive vessels. Vessels’ areas and branching indexes were higher among the chorioallantoic membranes with polyp implants and controls than among those with healthy mucosa implants. Nasal polyp presents differential angiogenic induction that impacts tissue growth.

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Funding

the authors declare that they received no funding for this study.

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Authors and Affiliations

  1. ENT Research Lab, Department of Otorhinolaryngology – Head and Neck Surgery, Federal University of São Paulo – UNIFESP, São Paulo, SP, Brazil

    Hallyson Andre Nascimento de Góes, Jônatas Bussador do Amaral & Rogério Pezato

  2. Centre of Heart Lung Innovation, University of British Columbia, Vancouver, BC, Canada

    Masih Sarafan, Andrew Thamboo & Rogério Pezato

  3. General Physics Department, Institute of Physics, Faculty of Pharmaceutical Sciences, University of São Paulo – USP, São Paulo, SP, Brazil

    Joyce dos Anjos Almeida & Lígia Ferreira Gomes

  4. Department of Ophthalmology and Otorhinolaryngology, University of São Paulo – USP, São Paulo, SP, Brazil

    Richard Louis Voegels & Rogério Pezato

  5. Marine Biology Department – Oceanographic Institute, University of São Paulo – USP, São Paulo, SP, Brazil

    Leandro Ticlia de la Cruz

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  1. Hallyson Andre Nascimento de Góes
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  2. Masih Sarafan
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  9. Rogério Pezato
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Correspondence to Rogério Pezato.

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de Góes, H.A.N., Sarafan, M., do Amaral, J.B. et al. Differential Angiogenic Induction Impacts Nasal Polyp Tissue Growth. Indian J Otolaryngol Head Neck Surg 75 (Suppl 1), 893–900 (2023). https://doi.org/10.1007/s12070-023-03469-y

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