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Expressions of matrix metalloproteinase-7 and matrix metalloproteinase-14 associated with the activation of extracellular signal-regulated kinase1/2 in human brain gliomas of different pathological grades

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Abstract

The purpose of this study is to determine whether the expressions of MMP-7 and MMP-14 are associated with the ERK 1/2 signaling pathway in human brain gliomas of different pathological grades. Immunohistochemistry and western blot methods were used to determine the expressions of MMP-7, MMP-14 and the phosphorylation status of ERK1/2 in 73 cases of human brain glioma specimens and two cases of normal brain tissues. Results indicated that the protein expression levels of MMP-7, MMP-14 and ERK1/2 phosphorylation level were all elevated with the increasing pathological grades in brain glioma tissues, and correlation assay indicated that the level of ERK1/2 phosphorylation was positively correlated with protein expression levels of MMP-7 and MMP-14 in gliomas of different pathological grades respectively. Moreover, the impact of ERK1/2 inhibitor U0126 on the expressions of MMP-7 and MMP-14 was examined in human U87 glioma cells by western blot analysis. The expressions of MMP-7 and MMP-14 were significantly decreased in human U87 glioma cells after treatment with ERK1/2 inhibitor U0126. The above results suggest that the expressions of MMP-7 and MMP-14 may be associated with activation of ERK1/2 signaling pathway in human brain gliomas of different pathological grades.

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Acknowledgments

This work was supported by the Natural Science Foundation of China, under contract numbers 30800451, 30872656, 30700861, 30670723, and 30973079; Scientific and Technological Research Projects in Colleges and Universities of Liaoning Province, number 2008850; the special fund for Scientific Research of Doctor-degree Subjects in Colleges and Universities, number 20092104110015; and Scientific and Technological Planning Projects of Shenyang, numbers 1072033-1-00 and 1081266-9-00.

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Authors and Affiliations

  1. Department of Neurobiology, College of Basic Medicine, China Medical University, 110001, Shenyang, Liaoning Province, People’s Republic of China

    Hui **e, Yi-xue Xue, Li-bo Liu & ** Wang

  2. Department of Neurosurgery, Sheng**g Hospital of China Medical University, 110004, Shenyang, Liaoning Province, People’s Republic of China

    Yun-hui Liu & Hao-qiang Ying

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  1. Hui **e
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  2. Yi-xue Xue
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  3. Li-bo Liu
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  4. ** Wang
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Correspondence to Yi-xue Xue.

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Hui **e, Yi-xue Xue contributed equally to this work.

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**e, H., Xue, Yx., Liu, Lb. et al. Expressions of matrix metalloproteinase-7 and matrix metalloproteinase-14 associated with the activation of extracellular signal-regulated kinase1/2 in human brain gliomas of different pathological grades. Med Oncol 28 (Suppl 1), 433–438 (2011). https://doi.org/10.1007/s12032-010-9660-7

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  • DOI: https://doi.org/10.1007/s12032-010-9660-7

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