Abstract
The protein kinase C (PKC) is known to be a critical component in the signaling cascades that lead to astrocyte-activation. To further understand the mechanism of PKC signaling in astrocyte-activation, we investigated the effect of SSeCKS, a PKC substrate, on LPS-induced cytokine expression in astrocytes by RT-PCR and enzymelinked immunosorbent assay. Exposure of the cells to LPS induced rapid translocation of SSeCKS to the perinuclear sides, ERK activation and pronounced TNF-α production, which can be inhibited by the PKC inhibitor Gö6983. By using siRNA knockdown of SSeCKS expression, LPS-induced signaling events were partly inhibited, including ERK activation, inducible TNF-α biosynthesis and secretion. These results suggest that SSeCKS is involved in the LPS-induced TNF-α expression in astrocytes mediated by PKC.
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Lin-lin Sun and Chun Cheng contributed equally to this work.
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Sun, L.l., Cheng, C., Liu, H.o. et al. Src suppressed C kinase substrate regulates the lipopolysaccharide-induced TNF-α biosynthesis in rat astrocytes. J Mol Neurosci 32, 16–24 (2007). https://doi.org/10.1007/s12031-007-0003-x
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DOI: https://doi.org/10.1007/s12031-007-0003-x