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Glycan analysis of Lamin A/C protein at G2/M and S phases of the cell cycle

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Abstract

During mitosis, phosphorylation and dephosphorylation of lamins triggers the nuclear envelope disassembly/assembly. However, it hasn’t been known whether lamin proteins undergo any modification other than phosphorylation during the cell cycle. Glycosylation of lamin proteins is one of the less studied post-translational modification. Glycosylation and phosphorylation compete for the same positions and interplay between two modifications generate a post-translational code in the cell. Based on this, we hypothesized that glycosylation of lamin A/C protein may be important in the regulation of the structural organization of the nuclear lamina during interphase and mitosis. We analysed the glycan units of lamin A/C protein in lung carcinoma cells synchronized at G2/M and S phases via CapLC-ESI-MS/MS. Besides, the outermost glycan units were determined using lectin blotting and gold-conjugated antibody and lectin staining. TEM studies also allowed us to observe the localization of glycosylated lamin A/C protein. With this study, we determined that lamin A/C protein shows O-glycosylation at G2/M and S phases of the cell cycle. In addition to O-GlcNAcylation and O-GalNAcylation, lamin A/C is found to be contain Gal, Fuc, Man, and Sia sugars at G2/M and S phases for the first time. Having found the glycan units of the lamin A/C protein suggests that glycosylation might have a role in the nuclear organization during the cell cycle.

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Acknowledgements

This work was supported by the Ege University Scientific Research Projects Coordination (grant number 17-FEN-031).

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  1. Faculty of Science, Department of Biology, Molecular Biology Section, Ege University, Izmir, Turkey

    Ecem Şener Uslupehlivan, Remziye Deveci, Umut Şahar & Savaş İzzetoğlu

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E.Ş.U. performed all the experiments and wrote the manuscript, R.D. performed the TEM preparation studies, U.Ş. performed the CapLC-ESI-MS/MS analysis, S.İ. obtained the TEM micrographs and supervised the project. All authors reviewed the manuscript.

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Correspondence to Savaş İzzetoğlu.

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Şener Uslupehlivan, E., Deveci, R., Şahar, U. et al. Glycan analysis of Lamin A/C protein at G2/M and S phases of the cell cycle. Cell Biochem Biophys 80, 689–698 (2022). https://doi.org/10.1007/s12013-022-01102-3

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  • DOI: https://doi.org/10.1007/s12013-022-01102-3

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