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Strontium Attenuates LPS-Induced Inflammation via TLR4/MyD88/NF-κB Pathway in Bovine Ruminal Epithelial Cells

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Abstract

Subacute ruminal acidosis (SARA) is a common nutritional metabolic disease in ruminants that causes significant economic losses to dairy farming. Strontium (Sr) is known to be involved in bone metabolism and exhibits potent anti-inflammatory effects. To evaluate the effect of Sr on inflammation in bovine ruminal epithelial cells, a model of LPS-induced inflammation was established in this study, and the cell viability of bovine ruminal epithelial cells was measured using CCK-8. The production of pro-inflammatory cytokines was measured by ELISA and real-time PCR, respectively. The related proteins of the TLR4/MyD88/NF-κB pathway were assayed through Western blotting, and the fluorescence of p-p65 and p-IκB were assayed by immunofluorescence. Molecular docking of Sr and TLR4/MyD88/NF-κB pathway-related proteins was performed using MIB2 (http://bioinfo.cmu.edu.tw/MIB2/). Results showed that after treatment for 24 h, the cell viability was decreased at the high concentration of Sr (≥ 10 mmol/L). Sr significantly decreased the production of TNF-α, IL-1β, and IL-6, downregulated the related proteins expression of the TLR4/MyD88/NF-κB pathway, and reduced the fluorescence levels of p-p65 and p-IκB. The NF-κB pathway inhibitor PDTC and molecular docking further revealed that Sr reduced LPS-induced pro-inflammatory cytokines production via the TLR4/MyD88/NF-κB pathway. These results suggest that Sr reduces LPS-induced pro-inflammatory cytokines production via the TLR4/MyD88/NF-κB pathway, thereby exerting an anti-inflammatory effect in bovine ruminal epithelial cells, providing a basis for Sr in the treatment of bovine rumen acidosis disease.

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Acknowledgements

The authors would like to thank the support of the China National Natural Science Foundation and Department of Science and Technology of Shaanxi Province and thank the editors and reviewers for their critical reading and constructive suggestions.

Funding

This work was supported by the National Natural Science Foundation of China (grant numbers 32273085, 32102742) and the Key Research and Development Program of Shaanxi (grant numbers 2021NY-022).

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  1. Panpan Tan and Jiaqi Yang contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Panpan Tan, Jiaqi Yang, Fanxuan Yi, Linshan Mei, Yazhou Wang, Chenxu Zhao, Baoyu Zhao & Jianguo Wang

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  1. Panpan Tan
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Contributions

All authors contributed to the study’s conception and design. The first draft of the manuscript was written by Panpan Tan and Jiaqi Yang. Material preparation, data collection and analysis were performed by Jiaqi Yang, Fanxuan Yi and Linshan Mei. The manuscript was revised by Yazhou Wang, Chenxu Zhao, Baoyu Zhao and Jianguo Wang. All authors read and approved the final manuscript.

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Correspondence to Baoyu Zhao or Jianguo Wang.

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Tan, P., Yang, J., Yi, F. et al. Strontium Attenuates LPS-Induced Inflammation via TLR4/MyD88/NF-κB Pathway in Bovine Ruminal Epithelial Cells. Biol Trace Elem Res (2023). https://doi.org/10.1007/s12011-023-03992-7

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