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Profiling of Circulatory Elements Reveals Alteration of Essential and Toxic Trace Metals in Crohn’s Disease

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Abstract

The status of essential and toxic trace metals in the blood of Crohn’s disease (CD) patients is unexplained. This study aimed to provide the first elemental profiling of the most recognized essential elements (Mn, Cu, Zn, Se) and selected toxic trace elements (As, Cd, Pb, and U) in sera and cell lysate (CL) samples of CD patients (n = 84). The results were compared with sex- and age-matched samples from the control group (CG). CD sera contained significantly higher levels of Mn, As, Cd, Pb, and U than did CG sera. An identical pattern, with the added inclusion of Cu (also higher in CD patients than in the CG), was obtained for CL samples. However, the most important finding was hypermanganesemia, which indicates that Mn could act as a toxic trace metal in CD. As, Cd, and U were the most significant toxic elements that showed antagonistic effects on the extrusion of essential Mn and Cu. Circulatory system screening markers for CD are hereby proposed (Mn/Cu, Mn/As, and Mn/Pb ratios). These three metal ratios were strongly and significantly correlated with F-Calprotectin levels, and deserve consideration as new markers of CD. The target metals and metal ratios should be taken into consideration as novel initiating and/or modifying factors for CD.

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Data Availability

Data and material are available from the corresponding author on reasonable request.

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Funding

This research was financially supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia, Contract Number: 451–03-9/2021–14/200288.

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Authors and Affiliations

  1. Faculty of Chemistry, University of Belgrade, Belgrade, Serbia

    Aleksandar Stojsavljević & Dragan Manojlović

  2. Department of Analytical Chemistry, Innovation Centre of the Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia

    Aleksandar Stojsavljević

  3. Clinic for Gastroenterology and Hepatology, University Clinical Center of Serbia, Belgrade, Serbia

    Aleksandra Sokić-Milutinović & Ljubiša Tončev

  4. Faculty of Medicine, University of Belgrade, Belgrade, Serbia

    Aleksandra Sokić-Milutinović & Branislav Rovčanin

  5. Center for Endocrine Surgery, University Clinical Center of Serbia, Belgrade, Serbia

    Branislav Rovčanin

  6. South Ural State University, Lenin prospect 76, Chelyabinsk, Russia

    Dragan Manojlović

Authors
  1. Aleksandar Stojsavljević
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  2. Aleksandra Sokić-Milutinović
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  3. Branislav Rovčanin
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  4. Ljubiša Tončev
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  5. Dragan Manojlović
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Contributions

A.S. designed the investigation, conducted the experimental study, analyzed the data, and wrote the first and last version of the paper; A.S.M. and Lj.T. collected clinical specimens; A.S.M., B.R. and D.M. participated in writing through reviewing and editing. All authors contributed critically to the draft and gave final approval for publication.

Corresponding author

Correspondence to Aleksandar Stojsavljević.

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Ethics Approval

Approval was obtained by the Local Ethical Board of the Clinic for Gastroenterology, Clinical Center of Serbia (Belgrade).

Consent to Participate

All patients and healthy blood donors voluntarily participated in this study, and written informed consent from all study participants was obtained according to the ethical standards defined by the Declaration of Helsinki.

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Not applicable.

Conflict of Interest

The authors declare no competing interests.

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Stojsavljević, A., Sokić-Milutinović, A., Rovčanin, B. et al. Profiling of Circulatory Elements Reveals Alteration of Essential and Toxic Trace Metals in Crohn’s Disease . Biol Trace Elem Res 200, 2572–2580 (2022). https://doi.org/10.1007/s12011-021-02862-4

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  • DOI: https://doi.org/10.1007/s12011-021-02862-4

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