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Solvent-Free Enzymatic Synthesis of Ethyl 3-Oxobutyrate Derivative: Characterization and Optimization of Reaction Condition

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Abstract

It was first found that porcine pancreatic lipase (PPL) could catalyze the Knoevenagel condensation of aromatic aldehydes and ethyl acetoacetate under solvent-free conditions in this paper. Under solvent-free conditions, the highest yield of PPL catalytic reaction was 99.38%, and the Z/E selectivity of the product was 3.93. In addition, the reaction conditions were optimized, and the factors affecting the product structure were studied.

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Funding

The Natural Science Foundation of Guangdong Province (Grant No.2018A030307022) and Special Innovation Projects of Common Universities in Guangdong Province (Grant No.2018KTSCX126).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, LingNan Normal University, Zhanjiang, 524048, China

    Yun Wang, Li-Mao Chen, **ao-qing Liao, Guo-huan Zhang & Jie Zhang

Authors
  1. Yun Wang
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  2. Li-Mao Chen
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  3. **ao-qing Liao
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  4. Guo-huan Zhang
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  5. Jie Zhang
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Contributions

Yun Wang: funding acquisition, project administration, supervision, visualization, writing—original draft, writing—review and editing, conceptualization, methodology. Li-Mao Chen: conceptualization, methodology, writing—original draft, writing—review and editing, validation. **ao-qing Liao: writing—review and editing, formal analysis. Guo-huan Zhang: formal analysis, writing—review and editing. Jie Zhang: writing—review and editing.

Corresponding author

Correspondence to Yun Wang.

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Wang, Y., Chen, LM., Liao, Xq. et al. Solvent-Free Enzymatic Synthesis of Ethyl 3-Oxobutyrate Derivative: Characterization and Optimization of Reaction Condition. Appl Biochem Biotechnol (2024). https://doi.org/10.1007/s12010-024-04981-9

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