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Effective Degradation of Brewer Spent Grains by a Novel Thermostable GH10 Xylanase

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Abstract

Brewer spent grains (BSGs) are one of the most abundant by-products in brewing industry. Due to microbiological instability and high perishability, the efficient degradation of BSGs is of environmental and economic importance. Streptomyces sp. F-3 could grow in the medium with BSGs as the only carbon and nitrogen source. Proteome mass spectrometry revealed that a GH10 xylanase SsXyn10A could be secreted in large quantities. SsXyn10A showed optimum activity at pH 7.0 and 60 °C. SsXyn10A exhibited excellent thermostability which retained approximately 100% and 58% after incubation for 5 h at 50 and 60 °C. SsXyn10A displayed high activity to beechwood xylan (BX) and wheat arabinoxylan (WAX). SsXyn10A is active against xylotetracose (X4), xylopentose (X5), and xylohexose (X6) to produce main products xylobiose (X2) and xylotriose (X3). Ssxyn10A showed synergistic effects with commercial cellulase on BSGs hydrolyzing into soluble sugar. In addition, the steam explosion pretreatment of BSGs as the substrate produced twice as much reducing sugar as the degradation of the original substrate. This study will contribute to efficient utilization of BSGs and provide a thermostable GH10 xylanase which has potential application in biomass hydrolysis.

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Data availability

The data generated or analyzed during this study are included in this published article and its supplementary information files. Further datasets used and analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was funded by the Open Research Fund of State Key Laboratory of Biological Fermentation Engineering of Beer (K202005), The National Natural Science Foundation of China (32100022), and the Key Research and Develop Program of Shandong Province (2020CXGC010601).

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Authors and Affiliations

  1. State Key Laboratory of Biological Fermentation Engineering of Beer, Tsingtao Brewery Co. Ltd, 56 Dengzhou Road, Shibei District, Qingdao, Shandong, 266000, China

    Shuxia Huang, Peng Yan, Hua Yin & Junhong Yu

  2. State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, 72 Binhai Road, Jimo District, Qingdao, Shandong, 266237, China

    Mengyu Liu, **uyun Wu & Lushan Wang

Authors
  1. Mengyu Liu
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  2. Shuxia Huang
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  3. Peng Yan
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  4. Hua Yin
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  5. Junhong Yu
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  6. **uyun Wu
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  7. Lushan Wang
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Contributions

ML performed the experiments and analyzed the data. SH and PY analyzed the data. HY and JY modified the article. XW conceived the study design and wrote the manuscript. LW edited the paper. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Junhong Yu or **uyun Wu.

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Liu, M., Huang, S., Yan, P. et al. Effective Degradation of Brewer Spent Grains by a Novel Thermostable GH10 Xylanase. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04779-1

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