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Utilization of Starch-Enriched Brewery (Rice Wine) Waste for Mixotrophic Cultivation of Ettlia Sp. YC001 Used in Biodiesel Production

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Abstract

Starch-enriched brewery waste (SBW), an unexplored feedstock, was investigated as a nutritious low-cost source for the mixotrophic cultivation of Ettlia sp. YC001 for biodiesel production. Stirring, autoclaving, and sonication were assessed for the SBW, in conjunction with pH. Stirring at 55 °C was found to be the best, in terms of the effectiveness of starch hydrolysis and yeast disintegration as well as cost. The treated solutions were found to support the mixotrophic growth of microalgae: 20 g/L of glucose medium resulted in the highest biomass production of 9.26 g/L and one with 10 g/L of glucose showed the best lipid productivity of 244.2 mg/L/day. The unsaturated fatty acids increased in the resulting lipid and thus quality well suited for the transportation fuel. All these suggested that SBW, when treated properly, could indeed serve as a cheap feedstock for microalgae-based biodiesel production.

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Acknowledgements

This work is financially supported by Grant KK-1605 from the Korea Institute of Toxicology. This work is also financially supported by Korea Minister of Ministry of Land, Infrastructure and Transport (MOLIT) as U-City Masters and Doctor Course Grant Program.

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Authors and Affiliations

  1. Department of Civil and Environmental Engineering, KAIST, 291 Daehakno, Yuseong-gu, Daejeon, 34141, Republic of Korea

    Yeji Kam, Mina Sung, Hoon Cho & Jong-In Han

  2. Gyeongnam Department of Environmental Toxicology & Chemistry, Korea Institute of Toxicology, **ju, 52834, Republic of Korea

    Chang-Min Kang

  3. Future Environmental Research Center, Korea Institute of Toxicology, **ju, 52834, Republic of Korea

    Jungmin Kim

  4. Human and Environmental Toxicology Program, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea

    Jungmin Kim

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  1. Yeji Kam
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Correspondence to Jong-In Han.

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Kam, Y., Sung, M., Cho, H. et al. Utilization of Starch-Enriched Brewery (Rice Wine) Waste for Mixotrophic Cultivation of Ettlia Sp. YC001 Used in Biodiesel Production. Appl Biochem Biotechnol 183, 1478–1487 (2017). https://doi.org/10.1007/s12010-017-2515-3

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  • DOI: https://doi.org/10.1007/s12010-017-2515-3

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