Abstract
In the present work, we evaluated the mineral composition of three sugarcane varieties from different areas in northeast Brazil and their influence on the fermentation performance of Saccharomyces cerevisiae. The mineral composition was homogeneous in the different areas investigated. However, large variation coefficients were observed for concentrations of copper, magnesium, zinc and phosphorus. Regarding the fermentation performances, the sugarcane juices with the highest magnesium concentration showed the highest ethanol yield. Synthetic media supplemented with magnesium also showed the highest yield (0.45 g g−1) while the excess of copper led to the lowest yield (0.35 g g−1). According to our results, the magnesium is the principal responsible for the increase on the ethanol yield, and it also seems to be able to disguise the inhibitory effects of the toxic minerals present in the sugarcane juice.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12010-014-1258-7/MediaObjects/12010_2014_1258_Fig1_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12010-014-1258-7/MediaObjects/12010_2014_1258_Fig2_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12010-014-1258-7/MediaObjects/12010_2014_1258_Fig3_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12010-014-1258-7/MediaObjects/12010_2014_1258_Fig4_HTML.gif)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs12010-014-1258-7/MediaObjects/12010_2014_1258_Fig5_HTML.gif)
Similar content being viewed by others
References
Basso, L. C., Basso, T. O., & Rocha, S. N. (2011). Ethanol production in Brazil: the industrial process and Its impact on yeast fermentation. In M. A. S. Bernardes (Ed.), Biofuel production-recent developments and prospects (pp. 85–100). Rijeka: Intech.
Amorim, H. V., Basso, L. C., & Lopes, M. L. (2009). Sugar cane juice and molasses, beet molasses and sweet sorghum: composition and usage. In W. M. Ingledew, G. D. Austin, C. Kluhspies, & D. R. Kelsall (Eds.), The alcohol textbook (5th ed., pp. 39–46). Nottingham: Nottingham University Press.
Walker, G. M. (2004). Advances in Applied Microbiology, 54, 197–229.
Stehlik-Thomas, V., Zetic, V. G., Stanzer, D., Grba, S., & Vahcic, N. (2004). Food Technology and Biotechnology, 42, 115–120.
Siverio, J. M. (2002). FEMS Microbiology Review, 26, 277–284.
Jones, R. P., & Greenfield, P. F. (1994). Process Biochemistry, 4, 48–59.
Youatt, J. (1993). Critical Reviews in Microbiology, 19, 83–97.
Vasconcelos, J. N. (1987). Brasil Açucareiro, 105, 41–48.
Courchesne, W. E., Vlasek, C., Klukovich, R., & Coffee, S. (2011). Archives of Microbiology, 193, 323–334.
De Lucena, R. M., Elsztein, C., Simões, D. A., & Morais, M. A., Jr. (2012). Journal of Applied Microbiology, 113, 629–640.
Cyert, M. S. (2003). Biochemical and Biophysical Research Communications, 311, 1143–1150.
Zhao, X. Q., & Bai, F. W. (2011). Journal of Biotechnology, 158, 176–183.
Magonet, E., Hayen, P., Delforge, D., Delaive, E., & Remacle, J. (1992). Journal of Biochemistry, 287, 361–365.
Berg, J. M., Tymoczko, J. L., & Stryer, L. (2002). Biochemistry (5th ed., p. 270). New York: WH Free-man and company. 465, 687.
Jones, R. P., & Gadd, G. M. (1990). Enzyme and Microbial Technology, 12, 1–17.
De Freitas, J., Wintz, H., Kim, J. H., Poynton, H., Fox, T., & Vulpe, C. (2003). Biometals, 16, 97–185.
Blackwell, K. J., Tobin, J. M., & Avery, S. V. (1997). Applied Microbiology and Biotechnology, 47, 180–184.
Pas, M., Piskur, B., Sustaric, M., & Raspor, P. (2007). Bioresource Technology, 98, 1622–1628.
Philpott, C. C., & Protchenko, O. (2008). Eukaryotic Cell, 7, 7–20.
Haug, A. (1984). Critical Reviews in Plant Sciences, 1, 345–373.
Oliveira, R. P. S., Torres, B. R., Zilli, M., Marques, D. A. V., Basso, L. C., & Converti, A. (2009). Archives of Environmental Contamination and Toxicology, 57, 488–494.
De Souza-Liberal, A. T., da Silva, F. E. A., de Morais, J. O. F., Simões, D. A., & Morais, M. A., Jr. (2005). Letters in Applied Microbiology, 40, 19–23.
De Souza-Liberal, A. T., Basílio, A. C. M., Brasileiro, B. T. R. V., Silva- Filho, E. A., Simões, D. A., & Morais, M. A., Jr. (2007). Journal of Applied Microbiology, 102, 538–547.
Basílio, A. C. M., Araújo, P. R. L., Morais, J. O. F., Silva-Filho, E. A., Morais, M. A., Jr., & Simões, D. A. (2008). Current Microbiology, 56, 322–326.
Lucena, B. T. L., Santos, B. M., Moreira, J. L. S., Moreira, A. P. B., Nunes, A. C., Azevedo, V., Miyoshi, A., Thompson, F. L., & Morais, M. A., Jr. (2010). BMC Microbiology, 10, e298.
EMBRAPA – Empresa Brasileira de Pesquisa Agropecuária. Centro Nacional de Pesquisa de Solos. (2006). Sistema brasileiro de classificação de solos. Brasília: Embrapa Produção de Informação.
Thomas, R. L., Shearrd, R. W., & Moyer, J. R. (1967). Agronomy Journal, 59, 240–243.
EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária. (1999). Manual de análises químicas de solos, plantas e fertilizantes. Brasília: Informática Agropecuária.
Silva-Filho, E. A., Santos, S. K. B., Resende, A. M., Morais, J. O. F., Morais, M. A., Jr., & Simões, D. A. (2005). Antonie Van Leeuwenhoek, 88, 13–23.
Basso, L. C., de Amorim, H. V., de Oliveira, A. J., & Lopes, M. L. (2008). FEMS Yeast Research, 8, 1155–1163.
De Souza, R. B., Santos, B. M., De Souza, R. F. R., Silva, P. K. N., Lucena, B. T. L., & Morais, M. A., Jr. (2012). Journal of Industrial Microbiology and Biotechnology, 39, 1645–1650.
Silva, F. A. S., & Azevedo, C. A. V. (2002). Revista Brasileira de Produtos Agroindustriais, 4, 71–78.
Pereira, L. P., Bassi, A. P. G., Avansini, S. H., Barbosa-Neto, A. G., Brasileiro, B. T. R. V., Ceccato-Antonini, S. R., & Morais, M. A., Jr. (2012). Antonie Van Leeuwenhoek, 101, 529–539.
De Barros Pita, W., Leite, F. C. B., De Souza Liberal, A. T., Simões, D. A., & De Morais, M. A., Jr. (2011). Antonie Van Leeuwenhoek, 100, 99–107.
Karamushka, V. I., & Gadd, G. M. (1994). FEMS Microbiology Letters, 122, 33–38.
Magasanik, B. (1992). Gene Expression, 2, 283–317.
Chandrasena, G., & Walker, G. M. (1997). Journal of the American Society of Brewing Chemists, 55, 24–29.
Schreier, B., & Hocker, B. (2010). Biochemistry, 49, 7582–7589.
Bollenback, T., & Nowak, T. (2001). Biochemistry, 40, 13097–13106.
König, S. (1998). Biochemica et Biophysica Acta, 1385, 271–286.
Tun, N. M., O’Doherty, P. J., Perrone, G. G., Bailey, T. D., Kersaitis, C., & Wu, M. J. (2013). Metallomics, 5, 1068–1075.
Cyert, M. S., & Philpott, C. C. (2013). Genetics, 193, 677–713.
Azenha, M., & Vasconcelos, M. T. (2000). Journal of Bioscience and Bioengineering, 90, 163–167.
Bleackey, M. R., & MacGillivray, R. T. (2011). Biometals, 24, 785–809.
Acknowledgments
The authors are grateful to all distilleries and farms that provided sugarcanes and fermentation samples for the experimental work. This work was supported by grants from the National Council of Technological and Scientific Development (CNPq) and the Bioethanol Research Network of the State of Pernambuco (CNPq-FACEPE/PRONEM).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
de Souza, R.B., de Menezes, J.A.S., de Souza, R.d.F.R. et al. Mineral Composition of the Sugarcane Juice and Its Influence on the Ethanol Fermentation. Appl Biochem Biotechnol 175, 209–222 (2015). https://doi.org/10.1007/s12010-014-1258-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-014-1258-7