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A bicistronic transgene system for genetic modification of Parthenium argentatum

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Abstract

Parthenium argentatum (guayule) was transformed with a bicistronic transgene containing a viral 2A cleavage sequence. The transgene includes the coding sequences of two key enzymes of the mevalonate pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and farnesyl pyrophosphate synthase (FPPS), involved in rubber biosynthesis. The viral 2A peptide sequence located between the two transgenes allowed for their co-expression via the Arabidopsis CBF2 (C-Binding repeat Factor 2) cold-inducible promoter. We identified three independent transgenic lines expressing the bicistronic transgenes upon cold treatment and examined the rubber content in the in vitro guayule plants.

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Acknowledgements

This research was funded by the United States Department of Agriculture, Agricultural Research Service, under National Program 306: Quality and Utilization of Agricultural Products. We are especially grateful to Roger Thilmony for critically reviewing our manuscript.

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Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, Albany, CA, 94710, USA

    Grisel Ponciano, Niu Dong, Grace Chen & Colleen McMahan

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  1. Grisel Ponciano
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  2. Niu Dong
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  3. Grace Chen
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  4. Colleen McMahan
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Correspondence to Grisel Ponciano.

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Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer.

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Ponciano, G., Dong, N., Chen, G. et al. A bicistronic transgene system for genetic modification of Parthenium argentatum. Plant Biotechnol Rep 12, 149–155 (2018). https://doi.org/10.1007/s11816-018-0478-7

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