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Quantitative shotgun proteomic analysis of cold-stressed mature rice anthers

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Abstract

Rice crops are vulnerable to low temperatures. During development, the reproductive stage is particularly sensitive to cold exposure, which causes abnormal pollen development and a high degree of male sterility. In this study, shotgun proteomic analysis was used to analyze rice anthers containing pollen grains from a cold-tolerant variety, Dianxi 4. Protein expression was compared between normal anthers and anthers exposed to cold temperatures at the young microspore stage. In total, 3835 non-redundant proteins were identified in the rice anther. Of these, 441 proteins were differentially expressed between normal and cold-treated anthers. Pollen allergens, ATP synthase, actin, profilin, and β-expansin proteins were highly abundant, reflecting anther development, pollen germination, and pollen tube elongation. Starch and sucrose metabolic proteins such as α-amylase precursor and 4-α-glucanotransferase exhibited reduced expression after cold exposure. Among the proteins that exhibited increased expression after cold exposure, C2 domain proteins, and GRPs were identified as candidate signaling factors for mediation of the cold tolerance response. Through high-throughput proteomic analysis we were able to reveal proteomic changes against cold stress and suggest two signaling factors as the candidate genes.

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Acknowledgements

This work was supported by the National Institute of Crop Science of the Rural Development Administration, Korea [Grant number PJ01099903].

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Authors and Affiliations

  1. Department of Crop Science, Konkuk University, Seoul, 05029, Republic of Korea

    Joohyun Lee, Yoonjung Lee & Mijeong Kim

  2. Department of Agricultural Science, Korea National Open University, Seoul, 03087, Republic of Korea

    Tae-Ho Ham

  3. Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang, 50424, Republic of Korea

    Su-Min Jo

  4. Department of Plant Bioscience, Pusan National University, 1268-50 Samnang**-ro, Miryang, 50463, Republic of Korea

    Soon-Wook Kwon

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  1. Joohyun Lee
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Correspondence to Soon-Wook Kwon.

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Lee, J., Lee, Y., Kim, M. et al. Quantitative shotgun proteomic analysis of cold-stressed mature rice anthers. Plant Biotechnol Rep 11, 417–427 (2017). https://doi.org/10.1007/s11816-017-0459-2

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