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Genome-Wide Identification of heat shock protein 10/60 Genes in Japanese Flounder (Paralichthys olivaceus) and Their Regulated Expression After Bacterial Infection

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Abstract

Heat shock proteins 10/60 (hsp10/60) are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful conditions. Besides the chaperone and housekee** functions, they are also known to be involved in immune response during bacterial infection. In this study, we identified and annotated 10 hsp10/60 genes through bioinformatic analysis in Japanese flounder (Paralichthys olivaceus). Among them one member of hsp10 (hspe) family and nine members of hsp60 (hspd) family were identified. Phylogenetic and selection pressure analysis showed that the hsp10/60 genes were evolutionarily constrained and their function was conserved. Besides, hsp10/60 genes were involved in different embryonic and larval stages and acted as the sentinel role in an unchallenged organism. In addition, we also observed the expression patterns of hsp10/60 genes after Edwardsiella tarda infection, for the first time in Japanese flounder. Eight out of 10 genes were differentially expressed after bacterial challenges, the significantly regulated expressions of flounder hsp10/60 genes after bacterial infections suggested their involvement in immune response in flounder. Our results provide valuable information for clarifying the evolutionary relationship, and early insights of the immune functions of hsp10/60 genes in Japanese flounder.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (No. 2018YFD0900 601) and the Natural Science Foundation of Shandong Province (No. ZR2017MC072).

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  1. Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Weijie Yan, Yingjie Qiao, Yuxiang Liu, Quanqi Zhang & Xubo Wang

  2. College of Life Sciences, Yantai University, Yantai, 264005, China

    **umei Liu

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Yan, W., Qiao, Y., Liu, Y. et al. Genome-Wide Identification of heat shock protein 10/60 Genes in Japanese Flounder (Paralichthys olivaceus) and Their Regulated Expression After Bacterial Infection. J. Ocean Univ. China 20, 886–896 (2021). https://doi.org/10.1007/s11802-021-4662-y

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