Abstract
Red algae represents an important marine bioresource. One high-value utilization of red algae is the production of agaro-oligosaccharides which have many positive biological effects. However, the lack of an efficient production route seriously limits the application of agaro-oligosaccharides. In this study, we established a green route that combines chemical liquefaction and enzymatic catalysis for the efficient production of agaro-oligosaccharides, and we used the production of neoagarotetraose (NA4) as an example. Agarose (150 g L−1) liquefaction by citric acid was controlled with respect to two targets: a 100% liquefaction rate and a high average degree of polymerization (> 4) of the liquesced agaro-oligosaccharides, which were then catalyzed by β-agarase into an oligosaccharides mixture with a high concentration of NA4 (30.8gL−1) in a 1-L reaction volume. After purification, we obtained 25.5g of NA4 with a purity of 92%. This work establishes an easy route for the efficient production of pure agaro-oligosaccharides from agarose.
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Acknowledgements
This work was supported by the National Key R&D Program of China (No. 2018YFC0311200), the Fundamental Research Funds for the Central Universities (No. 2019 41002), and the Taishan Scholars Project (No. tsqn2018 12020).
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Jiang, C., Liu, Z., Liu, J. et al. Applying Both Chemical Liquefaction and Enzymatic Catalysis Can Increase Production of Agaro-Oligosaccharides from Agarose. J. Ocean Univ. China 19, 1371–1377 (2020). https://doi.org/10.1007/s11802-020-4439-8
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DOI: https://doi.org/10.1007/s11802-020-4439-8