Abstract
The habitat of the endemic, endangered, and medically important plant, Stachys cretica subsp. kutahyensis belongs to the family Lamiaceae, is threatened by human influence. This study used a shoot tip explant to standardize a simple and proper micropropagation system for relict endemic plant species. Murashige Skoog medium with different concentrations of gibberellic acid and juglone was used for in vitro germination. Seedlings from in vitro germinated plantlets were used as explant sources in tissue culture studies. The highest shoot average per explant (4.60 shoots) and shoot length (2.70 cm) was determined in MS medium with 2.0 mg/L 6-benzyl amino purine with 100% response in all treatments. This study used different rooting mediums: Murashige Skoog and modified Murashige Skoog media. The effects of Murashige Skoog and different concentrations of indole butyric acid, naphthaleneacetic acid, juglone, and modified MS, and different concentrations of juglone were investigated on rooting development. Elongated shoots were successfully rooted in the Mod MS medium with 10–7 M juglone. Rooted plantlets were gradually transferred to the soil by adapted external conditions. The transfer of S. cretica subsp. kutahyensis plants, adapted to the soil and field conditions (Tavşanlı/Nusretler), successfully carried out. This protocol can be used for conservation studies of endemic, endangered, and medicinally important plant species.
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The data that support the findings of this study are available on request from the corresponding author.
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Akın, B., Bingöl, N.A. & Bulman, G.C. A first approach for the micropropagation of threatened endemic subspecies of Stachys cretica subsp. kutahyensis. Biologia 79, 1653–1661 (2024). https://doi.org/10.1007/s11756-024-01640-6
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DOI: https://doi.org/10.1007/s11756-024-01640-6