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Screening for agroactive and bioactive metabolites production by actinobacteria isolated from rhizospheric soils

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Abstract

In this study, agroactive and bioactive compound production by actinobacteria isolated from rhizospheres of different plants was screened. Six of the forty-two isolates showed the highest production of different compounds and were identified by using molecular methods. In the primary and secondary screening antimicrobial activities were determined by inhibition zones against six bacterial and three fungal pathogens. Streptomyces labedae CYP25 showed the highest antibacterial activity in screening with extracts obtained from Actinomycetes Isolation Broth (AIB) against Staphylococcus aureus (18 mm); Streptomyces sp. CYP30 showed the highest antifungal activities in screening with ethyl acetate extracts obtained from Glucose Soybean Meal Broth against Candida albicans (60 mm) and Alternaria sp. D21 (52 mm) and showed the highest antifungal activities in screening with extracts obtained from International Streptomyces Project-3 (ISP-3) Broth against Aspergillus niger (16 mm). The highest indole acetic acid (IAA) production was showed by Nocardiopsis sp. CYP39. This isolate produced the highest amount of IAA (76.5 µg/mL) in the presence of 0.9% L-tryptophan. Thirty-two of forty-two isolates were identified as siderophore producers. The best hydrogen cyanide producer was Nocardiopsis sp. CYP15. Streptomyces moderatus CYP35 and Streptomyces chartreusis CYP19 constitute the highest halo-zone diameter on phosphate solubilizing activity tests.

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Abbreviations

IAA:

Indole acetic acid

AIA:

Actinomycetes Isolation Agar

AIB:

Actinomycetes Isolation Broth

GSB:

Glucose Soybean Meal Broth

SCB:

Starch Casein Broth

ISP:

International Streptomycetes Project

CAS:

Chromium Azurol S

HCN:

Hydrogen cyanide

PVKA:

Pkiovskaya’s Agar

PGPR:

Plant Growth Promoting Rhizobacteria

BLAST:

Basic Local Alignment Search Tool

NCBI:

National Center for Biotechnology Information

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Authors and Affiliations

  1. Faculty of Science, Biology Department, Basic and Industrial Microbiology Section, Ege University, Bornova- İzmir, Turkey

    Hüseyin Evlat, Sultan K. Toker & Ali Koçyiğit

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  1. Hüseyin Evlat
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  2. Sultan K. Toker
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  3. Ali Koçyiğit
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Contributions

This study was performed based on a Master of Science (MSc) thesis addressed to Hüseyin EVLAT. All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Hüseyin EVLAT, Sultan Kübra TOKER and Ali KOÇYİĞİT. Ali KOÇYİĞİT supervised the study.The first draft of the manuscript was written by Hüseyin EVLAT and all authors commented and make corrections on all versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ali Koçyiğit.

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Evlat, H., Toker, S.K. & Koçyiğit, A. Screening for agroactive and bioactive metabolites production by actinobacteria isolated from rhizospheric soils. Biologia 78, 187–200 (2023). https://doi.org/10.1007/s11756-022-01226-0

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