Abstract
Giant freshwater prawn, Macrobrachium rosenbergii, is one of the major cultivated crustacean species in China. In recent years, a slow growth syndrome (SGS) has severely influenced the production, and the exact cause has not been clearly determined. In the present study, normal and SGS prawns were collected in 2016, 2017, and 2020. One-way ANOVA analysis showed significant difference in all growth traits examined between normal and SGS groups (P < 0.05). Cathepsin L (CatL) DNA fragment was cloned and re-sequenced, leading to identification of 41 SNPs. Among these SNPs and another four SNPs located in crustacean hyperglycemic hormone (CHH) that showed association with M. rosenbergii growth in Vietnam populations, 24 SNPs including two from the CHH gene were selected for association analysis. Eight out of the 24 SNPs were polymorphic in our population, in which SNP CatL-322 showed significant association with SGS (FDR adjusted P < 0.05). Fisher’s exact test was further conducted, and two intronic SNPs, CatL-322 and CHH-2409, showed significant association with carapace length of M. rosenbergii, explaining about 15 % of phenotypic variation in total. The two SNPs were further screened in a second population, and solid evidence was obtained. We compared the growth of different genotypes at each locus and confirmed that T allele at CatL-322 locus and G allele at CHH-2409 locus were beneficial to growth performance (P < 0.05). These two SNPs potentially can be applied in marker assisted selection in future M. rosenbergii breeding programs.
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Abbreviations
- SGS:
-
slow growth syndrome
- CatL:
-
cathepsin L
- CHH:
-
crustacean hyperglycemic hormone
- MIH:
-
molt-inhibiting hormone
- SNP:
-
single nucleotide polymorphism
- PCR:
-
polymerase chain reaction
- gDNA:
-
genomic DNA
- HWE:
-
Hardy-Weinberg Equilibrium
- FDR:
-
false discovery rate
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Acknowledgements
We thank Qun Jiang and **aojian Gao for their help in sampling. This study was supported by the Major Fishery Science and Technology Projects in Jiangsu Province (D2017-3) and the National Natural Science Foundation of China (31702004).
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Our study on Giant freshwater prawn, Macrobrachium rosenbergii, was approved by the Animal Care and Use Committee of the Yangzhou University (ethical protocol code: YZUDWSY 2016-05-06), and all efforts were made to minimize suffering. For instance, sufficient dissolved oxygen was provided during the transport, and all experimental prawns were first anesthetized with MS-222 (2 g/L, Sigma) before sacrificing and handling.
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Du, X., Yan, X., Zhang, W. et al. A SNP in Cathepsin L is associated with carapace length trait in giant freshwater prawn Macrobrachium rosenbergii. Biologia 76, 3587–3593 (2021). https://doi.org/10.1007/s11756-021-00860-4
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DOI: https://doi.org/10.1007/s11756-021-00860-4