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Optimization of Lipase-Catalyzed Synthesis of Cetyl Octanoate in Supercritical Carbon Dioxide

  • Original Paper
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Journal of the American Oil Chemists' Society

Abstract

Cetyl octanoate, a wax ester of 24 carbons, is widely used in the cosmetic industry as a base oil. The current work focuses on lipase-catalyzed synthesis of cetyl octanoate in supercritical carbon dioxide (SC-CO2) by esterification of cetyl alcohol and octanoic acid. Three immobilized lipases were screened, and 15 reaction conditions were tested in order to find the combination for maximal yield. The results showed that Novozym® 435 was the best catalyst for the synthesis, and a reaction time of 20 min was adequate for a maximal yield. Response surface methodology (RSM) with a 3-factor-3-level Box-Behnken design was employed to evaluate the effects of synthesis parameters, including reaction temperature (35–75 °C), pressure (8.27–12.41 MPa), and enzyme amount (5–15% wt of cetyl alcohol). A model for the synthesis was developed and the optimum conditions could be predicted to be reaction pressure of 10.22 MPa, reaction temperature of 63.70 °C, and enzyme amount of 11.20%. An experiment was performed under this optimum condition and a yield of 99.5% was obtained. This experimental yield correlated well with the predicted value of yield (97.6%). We concluded that, in a SC-CO2 system, nearly 100% molar conversion of cetyl octanoate could be obtained by immobilized Novozym® 435 in a short reaction time (20 min) under the predicted optimal conditions.

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Authors and Affiliations

  1. Biotechnology Center, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung, 402, Taiwan

    Chia-Hung Kuo & Chwen-Jen Shieh

  2. Department of Bioindustry Technology, Da-Yeh University, 168 University Road, Chang-Hwa, 515, Taiwan

    Hen-Yi Ju & Shuan-Wei Chu

  3. Graduate Institute of Molecular Biology, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung, 402, Taiwan

    Jiann-Hwa Chen

  4. Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung, 402, Taiwan

    Chieh-Ming J. Chang & Yung-Chuan Liu

Authors
  1. Chia-Hung Kuo
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  2. Hen-Yi Ju
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  3. Shuan-Wei Chu
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  4. Jiann-Hwa Chen
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  5. Chieh-Ming J. Chang
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  6. Yung-Chuan Liu
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  7. Chwen-Jen Shieh
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Correspondence to Yung-Chuan Liu or Chwen-Jen Shieh.

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Kuo, CH., Ju, HY., Chu, SW. et al. Optimization of Lipase-Catalyzed Synthesis of Cetyl Octanoate in Supercritical Carbon Dioxide. J Am Oil Chem Soc 89, 103–110 (2012). https://doi.org/10.1007/s11746-011-1895-8

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  • DOI: https://doi.org/10.1007/s11746-011-1895-8

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