SpringerLink
Log in

1-MCP treatment modulated physiological, biochemical and gene expression activities of guava during low-temperature storage

  • Original Article
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Guava fruits remain biologically active even after harvest as they continue respiration and other physioligical activites. Being climacteric in nature, guava fruits senesce fast duringstorage. To extend the storage life of guava fruits, they were treated with 1-MCP 500 ppb and found that 1-MCP could extend the storage life of guava. 1-MCP-treated fruits retained greater fruit texture (2.17 N) with reduced respiration (54.57 CO2 mg/kg/h), ethylene (5.02 µl/kg/h) and pectin methylestaerase activity (1.21 units/ml) after 21 days of storage period when compared to untreated fruits. To determine the effects of 1-MCP on ripening, various physiological, biochemcial and gene expression parameters were studied. The results showed that 1-MCP delayed respiration and ethylene peak by maintaining the quality parameters like ascorbic acid content (129.24 mg/100 g), antioxidant activity (199.2 AAE/100 g), phenols (528.93 GAE/100 g) and sugar acid ratio (16.05% acid) in the fruits. RNA isolation protocol was developed using Tris saturated phenol and sodium dodecyl sulfate. This rapid protocol allowed us to isolate quality RNA from guava pulp. Further, RT-qPCR gene expression analysis of expansin and ACC synthase genes reveled that the texture and ethylene production were significantly down regulated with 1-MCP treatment. The present reserach work highlighted the effect of 1-MCP in extending the shelf life of guava fruits by regulating physiological and biochemical processes during friut ripening, and their validation through gene expression studies provide insights for manipulaiton of genes involved in climacteric fruit ripening.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Abreu JRD, Santos CDD, Abreu CMP, Pinheriro ACM, Correa AD (2012) Ripening pattern of guava cv. Pedro Sato Cienc Tecnol Aliment Campinas 32:344–350

    Article  Google Scholar 

  • Addai ZR, Abdullah A, Mutalib SA (2013) Influence of ripening stages on antioxidant properties of papaya fruit (Carica papaya L.). AIP Conf Proc 1571:696–701. https://doi.org/10.1063/1.4858736

    Article  CAS  Google Scholar 

  • Ahmad A, Mohd Ali Z, Zainal Z (2013) Delayed softening of papaya (Carica papaya L. cv. Sekaki) fruit by 1-methylcyclopropene during ripening at ambient and low temperature storage conditions. Aus J Crop Sci 7:750–757

    CAS  Google Scholar 

  • Ali Z, Chin LH, Lazan H (2004) A comparative study on wall degrading enzymes, pectin modifications and softening during ripening of selected tropical fruits. Plant Sci 167:317–327

    Article  CAS  Google Scholar 

  • AOAC (1990): Official Methods of Analysis of the Association of Official‟s Analytical Chemists, 14th Ed. Association of Official Analytical Chemist, Washington D.C., pp: 223–225, 992–995

  • Ashraf F, Asbi A (1993) Pectinesterase extraction from Guava. Pertanika J Trap Agric Sci 16:223–224

    Google Scholar 

  • Bhandari SR, Lee JG (2016) Ripening-dependent changes in antioxidants, color attributes, and antioxidant activity of seven tomato (Solanum lycopersicum L.) cultivars. J Anal Methods Chem 16:1–13

    Article  Google Scholar 

  • Blankenship SM, Dole JM (2003) 1-Methylcyclopropene: a review. Postharvest Biol Technol 28:1–25

    Article  CAS  Google Scholar 

  • Cao S, Zheng Y, Yang Z (2011) Effect of 1-MCP treatment on nutritive and functional properties of loquat fruit during cold storage. New Zealand J Crop Hortic Sci 39:61–70

    Article  CAS  Google Scholar 

  • Chandra JL, Chemma RM, Kalal MJ (2011) Biochemical analysis of guava fruits. J Chem Stu 15:59–65

    Google Scholar 

  • Faasema J, Alakali JS, Abu JO (2012) Effects of storage temperature on 1-methylcyclopropene-treated mango (Mangniferaindica) fruit varieties. J Food pro Preserv 1:7

    Google Scholar 

  • Harrison EP, McQueen-Mason SJ, Manning K (2001) Expression of six expansin genes in relation to extension activity in develo** strawberry fruit. J Exp Bot 52:1437–1446

    Article  CAS  PubMed  Google Scholar 

  • Hayama H, Ito A, Moriguchi T, Kashimura Y (2003) Identification of a new expansin gene closely associated with peach fruit softening. Postharvest Biol Technol 29:1–10

    Article  CAS  Google Scholar 

  • Hiwasa K, Rose JKC, Nakano R, Inaba A, Kubo Y (2003) Differential expression of seven a-expansin genes during growth and ripening of pear fruit. Physiol Plant 117:564–572

    Article  CAS  PubMed  Google Scholar 

  • Hossain MS, Ramachandraiah K, Hasan R, Chowdhury RI, Kanan KA, Ahmed S, Ali MA, Islam MT, Ahmed M (2021) Application of oxalic acid and 1-methylcyclopropane (1-Mcp) with low and high-density polyethylene on post-harvest storage of litchi fruit. Sustainability 13:3703. https://doi.org/10.3390/su13073703

    Article  CAS  Google Scholar 

  • Jhalegar J, Sharma RR, Pal RK, Sharma S (2012) Effect of 1-MCP on shelf-life and quality of kiwifruit stored under ambient conditions. Indian J Hortic 69:258–262

    Google Scholar 

  • Khademi O, Besada C, Mostafi Y, Salvador A (2014) Changes in pectin methylesterase, polygalacturonase, catalase and peroxidase activities associated with alleviation of chilling injury in persimmon by hot water and 1-MCP treatments. Sci Horti 179:191–197. https://doi.org/10.1016/j.scienta.2014.09.028

    Article  CAS  Google Scholar 

  • Kim Y, Brecht JK, Talcott ST (2007) Antioxidant phytochemical and fruit quality changes in mango (Mangifera indica L.) following hot water immersion and controlled atmosphere storage. Food Chem 105:1327–1334

    Article  CAS  Google Scholar 

  • Kolniak OJ, Wojdyło A, Markowski J, Siucinska K (2014) 1-Methylcyclopropene postharvest treatment and their effect on apple quality during long-term storage time. Eur Food Res Technol 239:603–612

    Article  Google Scholar 

  • Lata D, Aftab MA, Homa F, Ahmad MS, Siddiqui MW (2018) Effect of eco-safe compounds on postharvest quality preservation of papaya (Carica papaya L.). Acta Physiol Plant 40:8

    Article  Google Scholar 

  • Lee J, Jeong MC, Ku KH (2017) Chemical, physical, and sensory properties of 1-MCP-treated Fuji apple (Malusdomestica Borkh.) fruits after long-term cold storage. Appl Biol Chem 60:363–374

    Article  CAS  Google Scholar 

  • Li D, Zhou HW, Sonego L, Lers A, Lurie S (2001) Ethylene involvement in the cold storage disorder of ‘Flavortop’ nectarine. Postharvest Biol Technol 23:105–115

    Article  CAS  Google Scholar 

  • Li L, Shuai L, Sun J (2020) The role of 1-Methylcyclopropene in the regulation of ethylene biosynthesis and ethylene receptor gene expression in Mangifera indica L. (Mango Fruit). Food Sci Nutr 8:1284–1294

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lohani S, Trivedi PK, Nath P (2004) Changes in activities of cell wall hydrolases during ethylene-induced ripening in banana: effect of 1-MCP, ABA and IAA. Postharvest Biol Technol 31:119–126

    Article  CAS  Google Scholar 

  • Lopez JE, Flores-Gallegos AC, Torres-Leon C, Ramírez-Guzman KN, Martinez GA, Aguilar CN (2021) Guava (Psidiumguajava L.) fruit and valorization of industrialization by-products. Processes 9:1075. https://doi.org/10.3390/pr9061075

    Article  CAS  Google Scholar 

  • Manenoi A, Bayogan ERV, Thumdee S, Paull RE (2007) Utility of 1- methylcyclopropene as a papaya postharvest treatment. Postharvest Biol Technol 44:55–62

    Article  CAS  Google Scholar 

  • Nishiyama K, Guis M, Rose JKC, Kubol Y, Bennett KA, Lu W, Katol K, Ushijima K, Nakano Z, Inba A (2007) Ethylene regulation of fruit softening and cell wall disassembly in Charentais melon. J Exp Bot 58:1281–1290

    Article  CAS  PubMed  Google Scholar 

  • Obenland DM, Crisosto CH, Rose JKC (2003) Expansin protein levels decline with the development of mealiness in peaches. Postharvest Biol Technol 29:11–18

    Article  CAS  Google Scholar 

  • Pech JC, Bouzayen M, Latche A (2008) Climacteric fruit ripening: ethylene-dependent and independent regulation of ripening pathways in melon fruit. Plant Sci 175:114–120

    Article  CAS  Google Scholar 

  • Porat R, Weiss B, Zipori I, Dag A (2009) Postharvest longevity and responsiveness of guava varieties with distinctive climacteric behaviors to 1-methylcyclopropene. Hort Technol 19:580–585

    Article  CAS  Google Scholar 

  • Ranganna S (1986) Handbook of analysis and quality control for fruit and vegetable products. 2nd edn. Tata McGraw-Hill Pub. Co, New Delhi, India.

  • Rao DVS, Rao GK (2008) Controlled atmosphere storage of mango cultivars ‘Alphonso’and ‘Banganapalli’ to extend storage-life and maintain quality. J Hortic Sci Biotechnol 83:351–359

    Article  CAS  Google Scholar 

  • Rawan S, Bibi F, Khan AM, Khattak Z, Shah A, Iqbal M, Alamzeb S, Haq A, Kamal FA, Shah A, Naeem, Ali W (2017) Postharvest life of guava (Psidium guajavaL.) varieties as affected by storage intervals at room temperature. Pak J Agric Res 30:155-161

  • Reddy SVR, Sharma RR, Barthakur S, Srivastav M (2015) An efficient and rapid method for the isolation of RNA from different recalcitrant tissues of mango (Mangifera indica L.). J Hort Sci Biotech 90:285–290

    Article  CAS  Google Scholar 

  • Reddy SVR, Sharma RR, Srivastava M, Kaur C (2016) Effect of pre-harvest application of Salicylic acid on the postharvest behavior of ‘Amrapali’ mango fruits during storage. Indian J Hort 73(3):405–409

    Article  Google Scholar 

  • Reddy SVR, Sharma RR, Barthakur S (2017) Influence of 1-MCP on texture, related enzymes, quality and their relative gene expression in ‘Amrapali’mango (Mangiferaindica L.) fruits. J Food Sci Tech 54:4051–4059

    Article  CAS  Google Scholar 

  • Sachin AJ, Rao DVS, Ranjitha K, Vasugi C, Narayana CK, Ravishankar K (2021) Differential effects of postharvest application of ethylene inhibitors on guava stored under ambient conditions. J Environ Biol 42:1395–1404. https://doi.org/10.22438/jeb/42/5/MRN-1753

    Article  CAS  Google Scholar 

  • Sakhale BK, Gaikwad SS, Chavan RF (2018) Application of 1-methylcyclopropene on mango fruit (Cv. Kesar): potential for shelf-life enhancement and retention of quality. J Food Sci Technol 55:776–781

    Article  CAS  PubMed  Google Scholar 

  • Salunkhe DK, Desai BB (1984). Postharvest Biotech. Fruits. Boca Raton, FC CRC. 168.

  • Shivashankara KS, Jalikop SH, Roy TK (2010) Species variability for fruit antioxidant and radical scavenging abilities in mulberry. Int J Fruit Sci 10:1–12

    Article  Google Scholar 

  • Sisler EC, Serek M (1997) Inhibitors of ethylene responses in plants at the receptor level: recent developments. Physiol Plant 100:577–582

    Article  CAS  Google Scholar 

  • Sivakumar D, Deventer FV, Terry LA, Polanta GA, Korsten L (2012) Combination of 1-methylcyclopropene treatment and controlled atmosphere storage retains overall fruit quality and bioactive compounds in mango. J Sci Food Agric 92:821–830

    Article  CAS  PubMed  Google Scholar 

  • Trivedi PK, Nath P (2004) MaExp1, an ethylene-induced expansin from ripening banana fruit. Plant Sci 167:1351–1358

    Article  CAS  Google Scholar 

  • Watkins CB (2006) The use of 1-methylcyclopropene (1-MCP) on fruits and vegetables. Biotechnol Adv 4:389–409

    Article  Google Scholar 

  • Zhang Z, Huber DJ, Rao J (2013) Antioxidant systems of ripening avocado (Perseaamericana Mill.) fruit following treatment at the preclimacteric stage with aqueous 1-methylcyclopropene. Postharvest Biol Technol 76:58–64. https://doi.org/10.1016/j.postharvbio.2012.09.00

    Article  CAS  Google Scholar 

  • Zhong Q, **a W (2007) Effect of 1-methylcyclopropene and/or chitosan coating treatments on storage life and quality maintenance of Indian jujube fruit. LWT-Food Sci Technol 40:404–411

    Article  CAS  Google Scholar 

  • Zhu X, Ye L, Ding X, Gao Q, **ao S, Tan Q, Chen JHW, Li X (2019) Transcriptomic analysis reveals key factors in fruit ripening and rubbery texture caused by 1-MCP in papaya. BMC Plant Biol 19:309

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge P.G. School, ICAR-IARI, New Delhi, India for providing the Ph.D. fellowship for the first author. We also acknowledge ICAR-IIHR, Bengaluru for providing Laboratory facilities.

Author information

Authors and Affiliations

  1. Division of Postharvest Technology and Agricultural Engineering, ICAR-Indian Institute of Horticultural Research, Hesaraghatta, Bengaluru, 560089, India

    A. J. Sachin, D. V. Sudhakar Rao, K. Ranjitha, C. K. Narayana & S. Vijay Rakesh Reddy

  2. Division of Fruit Crops, ICAR-Indian Institute of Horticultural Research, Hesaraghatta, Bengaluru, 560089, India

    C. Vasugi

  3. Division of Basic Sciences, ICAR-Indian Institute of Horticultural Research, Hesaraghatta, Bengaluru, 560089, India

    Kundapura Ravishankar

Authors
  1. A. J. Sachin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. V. Sudhakar Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kundapura Ravishankar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Ranjitha
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. Vasugi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. K. Narayana
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. S. Vijay Rakesh Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. J. Sachin.

Additional information

Communicated by P. K. Nagar.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sachin, A.J., Rao, D.V.S., Ravishankar, K. et al. 1-MCP treatment modulated physiological, biochemical and gene expression activities of guava during low-temperature storage. Acta Physiol Plant 44, 125 (2022). https://doi.org/10.1007/s11738-022-03463-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11738-022-03463-x

Keywords

Search

Navigation