SpringerLink
Log in

Cloning and expression of genes related to the sucrose-metabolizing enzymes and carbohydrate changes in peach

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

To shed light on the relationship between sucrose metabolism and expression of genes related to sucrose-metabolizing enzymes, six genes encoding sucrose-metabolizing enzymes were isolated, and the levels of four main carbohydrates and related enzyme activities as well as the expression of these six genes were determined in fruits, leaves and phloem-enriched fraction throughout peach fruit development. Sucrose content in mature fruit ranked first followed by glucose, fructose and sorbitol in that order, while sorbitol was the highest and sucrose lowest in phloem-enriched fraction and leaves. Glucose and fructose had similar change patterns throughout fruit development. Cloning results reveal that the nucleotide sequences of the six genes have high similarity to corresponding genes isolated from other plants. In addition, the expression of these genes and the levels of related enzyme activities varied with tissue and stage of fruit development, suggesting a complexity in relationships between carbohydrates, enzymes activities and related gene expression. Sucrose phosphate synthase maybe a key enzyme involved in sucrose synthesis while sucrose synthase may mainly be responsible for sucrose synthesis in peach fruits at later stages of development. Further studies are needed to genetically and physiologically characterize these genes and enzymes in peach and to gain a better understanding of their functions and relationship with carbohydrate metabolism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Azcon-Bieto J (1983) Inhibition of photosynthesis by carbohydrates in wheat leaves. Plant Physiol 73:681–686

    Article  PubMed  CAS  Google Scholar 

  • Baroja-Fernandez E, Munoz FJ, Saikusa T, Rodriguez-Lopez M, Akazawa T, Pozueta-Romero J (2003) Sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants. Plant Cell Physiol 44:500–509

    Article  PubMed  CAS  Google Scholar 

  • Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611–622

    Article  PubMed  CAS  Google Scholar 

  • Chen CB, Zhu SH, Zhou J (2009) Improved CTAB method for total RNA extraction of mature Feicheng peach fruit. Shandong Agri Sci 5:102–104

    Google Scholar 

  • Cheng JS, Fan PG, Liang ZC, Wang YQ, Niu N, Li WD, Li SH (2009) Accumulation of end products in source leaves affects photosynthetic rate in peach via alteration of stomatal conductance and photosynthetic efficiency. J Amer Soc Hort Sci 134:667–676

    Google Scholar 

  • Dali N, Michaud D, Yelle S (1992) Evidence for the involvement of sucrose phosphate synthase in the pathway of sugar accumulation in sucrose-accumulating tomato fruits. Plant Physiol 99:434–438

    Article  PubMed  CAS  Google Scholar 

  • Dantas BF, Ribeiro LDS, Silva APD, Luz SRDS (2005) Foliar carbohydrates content and invertase activity in vines at Sao Francisco River Valley-Brazil. Rev Bras Frutic 27:198–202

    Article  Google Scholar 

  • Dunford S (1998) Translocation in the phloem. In: Taiz L, Zeigers E (eds) Plant physiology. Sinauer Associates, Sunderland

  • ElSayed AI, Ramadan MF, Komor E (2010) Expression of sucrose transporter (ShSUT1) in a Hawaiian sugarcane cultivar infected with Sugarcane yellow leaf virus (SCYLV). Physiol Mol Plant Path 75:56–63

    Article  CAS  Google Scholar 

  • Etienne C, Rothan C, Moing A, Plomion C, Bodenes C, Svanella-Dumas L, Cosson P, Pronier V, Monet R, Dirlewanger E (2002) Candidate genes and QTLs for sugar and organic acid content in peach [Prunus persica (L.) Batsch]. Theor Appl Genet 105:145–159

    Article  PubMed  CAS  Google Scholar 

  • Guo XF, Li SH, Liu GJ, Fu ZF, Li ST (2004) Seasonal changes in carbohydrate content and related enzyme activity in fruit and leaves of ‘Yanfengyihao’ peach variety. J Fru Sci 21:196–200

    Google Scholar 

  • Heim U, Weber H, Wobus U (1996) Cloning and characterization of full-length cDNA encoding sucrose phosphate synthase from faba bean. Gene 178:201–203

    Article  PubMed  CAS  Google Scholar 

  • Hubbard NL, Huber SC, Pharr DM (1989) Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in develo** muskmelon (Cucumis melo L.) fruits. Plant Physiol 91:1527–1534

    Article  PubMed  CAS  Google Scholar 

  • Hubbard NL, Phar DM, Huber SC (1990) Role of sucrose phosphate synthase in sucrose biosynthesis in ripening bananas and its relationship to the respiratory climacteric. Plant Physiol 94:201–208

    Article  PubMed  CAS  Google Scholar 

  • Huber SC, Huber JL (1996) Role and regulation of sucrose-phosphate synthase in higher plants. Annu Rev Plant Physiol Plant Mol Biol 47:431–444

    Article  PubMed  CAS  Google Scholar 

  • Ji XM, Van den Ende W, Van Laere A, Cheng SH, Bennett J (2005) Structure, evolution, and expression of the two invertase gene families of rice. J Mol Evol 60:615–634

    Article  PubMed  CAS  Google Scholar 

  • Koch KE (1996) Carbohydrated-modulated gene expression in plants. Annu Rev Plant Physiol Plant Mol Biol 47:509–540

    Article  PubMed  CAS  Google Scholar 

  • Krapp A, Stitt M (1995) An evaluation of direct and indirect mechanisms for the “sink-regulation” of photosynthesis in spinach: changes in gas exchanges, carbohydrates, metabolites, enzyme activities and steady-state transcript levels after cold-girdling source leaves. Planta 195:313–323

    Article  CAS  Google Scholar 

  • Kuhn C, Grof CPL (2010) Sucrose transporters of higher plants. Curr Opin Plant Biol 13:288–298

    Article  PubMed  Google Scholar 

  • Liesche J, Krügel U, He HX, Chincinska I, Hackel A, Kühn C (2011) Sucrose transporter regulation at the transcriptional, post-transcriptional and post-translational level. J Plant Physiol 168:1426–1433

    Article  PubMed  CAS  Google Scholar 

  • Lingle SE, Dunlap JR (1987) Sucrose metabolism in netted muskmelon fruit during development. Plant Physiol 84:386–389

    Article  PubMed  CAS  Google Scholar 

  • Lingle SE, Dyer JM (2001) Cloning and expression of sucrose synthase-1 cDNA from sugarcane. Plant Physiol 158:129–131

    Article  CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−∆∆CT method. Methods 25:402–408

    Article  PubMed  CAS  Google Scholar 

  • Lo Bianco R, Rieger M (2002) Partitioning of sorbitol and sucrose catabolism within peach fruit. J Am Soc Hortic Sci 127:115–121

    CAS  Google Scholar 

  • Lombardo VA, Osorio S, Borsani J, Lauxmann MA, Bustamante CA, Budde CO, Andreo CS, Lara MV, Fernie AR, Drincovich MF (2011) Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage. Plant Physiol 157:1696–1710

    Article  PubMed  CAS  Google Scholar 

  • Miron D, Schaffer AA (1991) Sucrose phosphate synthase, sucrose synthase, and invertase activities in develo** fruit of Lycopersicon esculentum Mill. and the sucrose accumulating Lycoper-sicon hirsutum Humb. and Bonpl. Plant Physiol 95:623–627

    Article  PubMed  CAS  Google Scholar 

  • Moing A (2000) Sugar alcohols as carbohydrate reserves in some higher plants. In: Gupta AK, Kaur N (eds) Carbohydrate reserves in plants-synthesis and regulation. Dev Crop Sci 26:337–358

  • Moing A, Carbonne F, Rashad MH, Gaudille`re JP (1992) Carbon fluxes in mature peach leaves. Plant Physiol 100:1878–1884

    Article  PubMed  CAS  Google Scholar 

  • Moing A, Svanella L, Rolin D (1998) Compositional changes during the fruit development of two peach cultivars differing in juice acidity. J Amer Soc Hort Sci 123:770–775

    CAS  Google Scholar 

  • Morandi B, Corelli Grappadelli L, Rieger M, Lo Bianco R (2008) Carbohydrate availability affects growth and metabolism in peach fruit. Physiol Plant 133:229–241

    Article  PubMed  CAS  Google Scholar 

  • Moriguchi T, Yamaki S (1988) Purification and characterization of sucrose synthase from peach (Prunus persica) fruit. Plant Cell Physiol 29:1361–1366

    CAS  Google Scholar 

  • Moscatelloa S, Famianib F, Proietti S, Farinelli D, Battistelli A (2011) Sucrose synthase dominates carbohydrate metabolism and relative growth rate in growing kiwifruit (Actinidia deliciosa, cv Hayward). Sci Hort 128:197–205

    Article  Google Scholar 

  • Nii N (1997) Changes of starch and sorbitol in leaves before and after removal of fruits from peach trees. Ann Bot 79:139–144

    Article  CAS  Google Scholar 

  • Niu J, Zhao JB, Wu BH, Li SH, Liu GJ, Jiang Q (2006) Sugar and acid contents in peach and nectarine derived from different countries and species. Acta Hort Sin 33:6–11

    CAS  Google Scholar 

  • Nonis A, Ruperti B, Falchi R, Casatta E, Enferadi ST, Vizzotto G (2007) Differential expression and regulation of a neutral invertase encoding gene from peach (Prunus persica): evidence for a role in fruit development. Physiol Plant 129:436–446

    Article  CAS  Google Scholar 

  • Paul MJ, Foyer CH (2001) Sink regulation of photosynthesis. J Exp Bot 52:1383–1400

    Article  PubMed  CAS  Google Scholar 

  • Robertson JA, Horvat RJ, Lyon BG, Meredith FI, Senter SD, Okie WR (1990) Comparison of quality characteristics of selected yellow- and white-fleshed peach cultivars. J Food Sci 55:1308–1311

    Article  Google Scholar 

  • Roitsch T, Gonza′lez MC (2004) Function and regulation of plant invertases: sweet sensations. Trends Plant Sci 9:606–613

    Article  PubMed  CAS  Google Scholar 

  • Rolland F, Moore B, Sheen J (2002) Sugar sensing and signaling in plants. Plant Cell 14:185–205

    Google Scholar 

  • Shen ZJ, Ma RJ, Yu ML, Cai ZX, Song HF, Li X (2007) Regularity analysis of main sugar and acid in fruit development of peach. Acta Agri Bor Occid Sin 22:130–135

    Google Scholar 

  • Tian HM, Kong QG, Feng YQ, Yu XY (2009) Cloning and characterization of a soluble acid invertase-encoding gene from muskmelon. Mol Biol Rep 36:611–617

    Article  PubMed  CAS  Google Scholar 

  • Tong ZG, Zhang Z, Wang FR (2007) Molecular cloning and characterization of a sucrose phosphate synthase gene from peach (Prunus persica). Submitted to the EMBL/GenBank/DDBJ databases

  • Tong ZG, Gao ZH, Wang F, Zhou J, Zhang Z (2009) Selection of reliable reference genes for gene expression studies in peach using real-time PCR. BMC Mol Biol 10:1–13

    Article  Google Scholar 

  • Verma AK, Upadhyay SK, Verma PC, Solomon S, Singh SB (2011) Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (Saccharum) cultivars. Plant Biol (Stuttg) 13:325–332

    Article  CAS  Google Scholar 

  • Vizzotto G, Pinton R, Varanini Z, Costa G (1996) Sucrose accumulation in develo** peach fruit. Physiol Plant 96:225–230

    Article  CAS  Google Scholar 

  • Wan SB, Zhang B, Zhan JC, Chen JY, Yin JY (2009) In Silico cloning and sequence analysis of phospholipase Dα gene from peach fruit. Agri Sci Chin 8:1293–1300

    Article  Google Scholar 

  • Wang YQ, Wu BH, Zhao JB, Jiang Q, Li SH (2008) Soluble sugar contents in fruits and leaves during fruit development and their relationship in peach cultivars of difference in fruit glucose/fructose. Sci Agri Sin 41:2063–2069

    CAS  Google Scholar 

  • Wibbe ML, Blanke MM (1995) Effects of defruiting on source-sink relationship, carbon budget, leaf carbohydrate content and eater use efficiency of apple trees. Physiol Plant 94:529–533

    Article  CAS  Google Scholar 

  • Winter H, Huber SC (2000) Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes. Crit Rev Biochem Mol Biol 35:253–289

    Article  PubMed  CAS  Google Scholar 

  • Wobus U, Weber H (1999) Sugars as signal molecules in plant seed development. Biol Chem 380:937–944

    Article  PubMed  CAS  Google Scholar 

  • Wrolstad RE, Shallenberger RS (1981) Free sugars and sorbitol in fruits: a compilation from the literature. J Assoc Off Anal Chem 64:91–103

    PubMed  CAS  Google Scholar 

  • Zhang YP, Wang C, Yu HP, Cai BH, Fang JG (2010) Screening of RNA extraction methods for various grapevine organs and tissues. Acta Agri Bor Occid Sin 19:135–140

    Google Scholar 

  • Zhang XM, Dou MA, Yao YL, Du LQ, Li JG, Sun GM (2011) Dynamic analysis of sugar metabolism in differ harvest seasons of pineapple (Ananas comosu L. (Merr.)). Afr J Biotechnol 10:2716–2723

    Article  CAS  Google Scholar 

  • Zhou R, Quebedeaux B (2003) Changes in photosynthesis and carbohydrate metabolism in mature apple leaves in response to whole plant source-sink manipulation. J Amer Soc Hort Sci 128:113–119

    CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to thank the Natural Science Foundation of China (No.30871681) and Postdoctoral Scientific Research Foundation of Jiangsu Province (No.1002024B) for financial Support. The authors also thank Prof. **ggui Fang for providing critical facilities for research as well as comments and suggestions for the manuscript.

Conflict of interest

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nan**g, 210014, People’s Republic of China

    Chunhua Zhang, Zhijun Shen, Ruijuan Ma & Mingliang Yu

  2. College of Horticulture, Nan**g Agricultural University, Nan**g, 210095, Weigang, People’s Republic of China

    Yan** Zhang, Jian Han & Nicholas Kibet Korir

Authors
  1. Chunhua Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhijun Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yan** Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ruijuan Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nicholas Kibet Korir
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mingliang Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mingliang Yu.

Additional information

Communicated by Y. Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, C., Shen, Z., Zhang, Y. et al. Cloning and expression of genes related to the sucrose-metabolizing enzymes and carbohydrate changes in peach. Acta Physiol Plant 35, 589–602 (2013). https://doi.org/10.1007/s11738-012-1100-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-012-1100-1

Keywords

Search

Navigation