Abstract
To understand the diversity of culturable fungi in soil at alpine sites, Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland of the Eastern Qilian Mountains were selected to investigate. Three methods, including traditional culturing, rDNA internal transcribed spacer (ITS) sequence analysis, and economical efficiency analysis, were carried out to estimate the diversity of soil culturable fungi of these three alpine shrublands. A total of 35 strains of culturable fungi were cultured by dilution plate technique and were analyzed by rDNA ITS sequence. The diversity indices such as species abundance (S), Shannon-Wiener index (H), Simpson dominance index (D), and Pielou evenness index (J) of Rhododendron fruticosa shrubland, Salix cupularis fruticosa shrubland, and Dasiphoru fruticosa shrubland were ranged between 16 and 17, 2.66–2.71, 0.92, 0.95–0.97 respectively. The results showed that the diversity of soil fungi were abundant in these three types of alpine shrub grasslands, while further study should be done to explore their potential value.
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Junzhong Zhang is a Research Assistant at Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, Southwest Forestry University, China. He received his M.S. and Ph. D. in grassland biodiversity at Gansu Agricultural University, China in 2007 and 2010, respectively. As a visiting scholar he studied at College of Environmental Sciences, Tsinghua University during September 2011 to July 2012. His research areas focus on environmental microorganisms and plant pathogens. Dr. Zhang has published over 10 papers, and one book.
Baiying Man is a teacher at Guangzhou University Sontan College, China. He holds his B.S. in life sciences from the Tianshui Normal University and M.S. in agriculture from Gansu Agricultural University, China in 2005 and 2008, respectively. His research areas focus on grassland microorganisms and plant endophytic bacteria. He has published five scientific papers on grassland microorganisms and plant endophytic bacteria.
Benzhong Fu received his B.S. in plant protection from Hubei Agricultural College, **gzhou, China in 2005, M.S. in plant pathology from Yunnan Agricultural University, China in 2009, and Ph.D. in plant pathology from Chinese Academy of Agricultural Sciences, China. He was a lecturer in Southwest Forestry University from July 2009 to September 2012. Since then, He employed in Hubei Engineering University, China. His current research interesting includes microbial molecular ecology, molecular plant-microbe interactions, and interaction between different microbes. Dr. Fu is the membership of Plant Pathology Society of Hubei Province, China.
Li Liu earned his M.S. in plant pathology from Fujian Agriculture and Forestry University, Fuzhou City, China in 2005. Now her main study field is the microbe of agriculture. She had worked in Mudanjiang Agriculture Institute, Heilongjiang Academy of Agriculture Science from August 2000 to August 2002. Now she worked in Southwest Forestry University, China. Her current research interest is microbe of agriculture.
Changzhi Han graduated from Nan**g Agricultural University, China in August 2010, and now he is a Ph.D. candidate in agronomy, specializing in plant pathology and research direction is fungal genetic and functional group. From August 2010, he worked at the Southwest Forestry University, China, and he is interesting in the forest fungal disease genetic and pathogenic gene function.
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Zhang, J., Man, B., Fu, B. et al. The diversity of soil culturable fungi in the three alpine shrub grasslands of Eastern Qilian Mountains. Front. Earth Sci. 7, 76–84 (2013). https://doi.org/10.1007/s11707-012-0345-8
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DOI: https://doi.org/10.1007/s11707-012-0345-8