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Glioma cell line proliferation controlled by different chemical functional groups in vitro

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Abstract

Glioma cell line C6 cultured on silicon surfaces modified by different chemical functional groups, including mercapto (-SH), carboxyl (-COOH), amino (-NH2), hydroxyl (-OH) and methyl (-CH3) groups, was studied here to investigate the influence of surface chemistry on the cell proliferation, adhesion and apoptosis. AFM confirmed the similar characteristic of different functional groups occupation. The adhering C6 exhibited morphological changes in response to different chemical functional groups. The C6 adhered to -COOH, -NH2, -OH and -CH3 surfaces and flattened morphology, while those on -SH surface exhibited the smallest contact area with mostly rounded morphology, which led to the death of cancer cells. The results of MTT assay showed that the -COOH and -NH2 groups promoted cell proliferation, while the -SH significantly inhibited the proliferation. Compared with other chemical functional groups, the -SH group exhibited its unique effect on the fate of cancer cells, which might provide means for the design of biomaterials to prevent and treat glioma.

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Authors and Affiliations

  1. **nyuan Institute of Medicine and Biotechnology, School of Life Science, Zhejiang Sci-Tech University, Hangzhou, 310018, China

    Su-Ju Xu & **ang-Dong Kong

  2. Institute of Regenerative Medicine and Biomimetic Materials, Department of Materials Science and Engineering, Tsinghua University, Bei**g, 100084, China

    Su-Ju Xu, Fu-Zhai Cui & **ao-Long Yu

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  1. Su-Ju Xu
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  2. Fu-Zhai Cui
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  3. **ao-Long Yu
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  4. **ang-Dong Kong
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Correspondence to **ang-Dong Kong.

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Xu, SJ., Cui, FZ., Yu, XL. et al. Glioma cell line proliferation controlled by different chemical functional groups in vitro . Front. Mater. Sci. 7, 69–75 (2013). https://doi.org/10.1007/s11706-013-0195-7

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  • DOI: https://doi.org/10.1007/s11706-013-0195-7

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