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Analysis of Brucella cellular fatty acids

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Abstract

To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAs information in ty** Brucella, 19 reference strains were subjected to CFAs study. After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylated and extracted to provide fatty acid methylesters for gas chromatography (GC) analysis. Based on the CFAs data matrix, a dendrogram of 19 reference strains was generated by SPSS11.5 software package. The results showed that 19 reference strains were divided into five clusters: cluster 1 included B. suis (bv. 1, 2, 3, 5) and B. ovis; cluster 2 included B. abortus (bv. 3, 4, 5, 6) and B. melitensis (bv. 1, 2, 3); cluster 3 included B. abortus (bv. 1, 2, 7, 9) and B. neotomae; cluster 4 was B. suis (bv. 4); and cluster 5 was B. canis. Ty** Brucella by GC analysis of CFAs is a good method to reflect drug resistance of Brucella, and the classification is beneficial for clinical therapy. It also provides a new result of ty** and demonstrates that the traditional classification is not completely reasonable. CFAs analysis may identify B. suis (bv. 4) and B. canis.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Shanxi Medical University, Taiyuan, 030001, China

    Zhen-**ang Zhao & Su-Zhen Hao

  2. Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Bei**g, 102206, China

    Zhen-**ang Zhao, Hai Jiang, Bu-Yun Cui, Hong-Yan Zhao, Dong-Ri Piao & Lan-Yu Li

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  1. Zhen-**ang Zhao
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Correspondence to Bu-Yun Cui.

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Both authors contributed equally to this work.

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Zhao, ZX., Jiang, H., Cui, BY. et al. Analysis of Brucella cellular fatty acids. Front. Med. China 4, 216–219 (2010). https://doi.org/10.1007/s11684-010-0037-2

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