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Rumex acetosella Inhibits Platelet Function via Impaired MAPK and Phosphoinositide 3-Kinase Signaling

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Abstract

Objective

To examine the antiplatelet and antithrombotic activity of Rumex acetosella extract.

Methods

Standard light aggregometry was used for platelet aggregation, intracellular calcium mobilization assessed using Fura-2/AM, granule secretion (ATP release) by luminometer, and fibrinogen binding to integrin αIIbβ3 detected using flow cytometry. Western blotting is carried out to determine the phosphorylation of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt signaling.

Results

Rumex acetosella displayed the ability to inhibit platelet aggregation, calcium mobilization, granule secretion, and fibrinogen binding to integrin αIIbβ3. Rumex acetosella has also down-regulated MAPK and PI3K/Akt phosphorylation (all P<0.01).

Conclusion

Rumex acetosella extract exhibits antiplatelet activity via modulating GPVI signaling, and it may protect against the development of platelet-related cardiovascular diseases.

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Acknowledgment

The present data is a part of Ph.D. dissertation of Bo-Ra Jeon. R. acetosella was provided by Dr. Seung-Eun Lee in Rural Development Administration (RDA), Republic of Korea.

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Authors and Affiliations

  1. Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Bo-Ra Jeon, Muhammad Irfan & Man Hee Rhee

  2. Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), Eumseong, 27709, Republic of Korea

    Seung Eun Lee & Jeong Hoon Lee

Authors
  1. Bo-Ra Jeon
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  2. Muhammad Irfan
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  3. Seung Eun Lee
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  4. Jeong Hoon Lee
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  5. Man Hee Rhee
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Contributions

Rhee MH and Jeon BR designed and conceptualized the study. Jeon BR and Irfan M performed experiments, and wrote the manuscript. Irfan M critically revised the manuscript. Lee SE, Lee JH, and Rhee MH technically assisted in the experiments and preparation of the manuscript. Rhee MH supervised the whole research work. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Man Hee Rhee.

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Conflict of Interest

Authors declare no conflict of interest.

Supported by a grant of National Research Foundation of Korea (No. 2018R1D1A1A09083797)

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Jeon, BR., Irfan, M., Lee, S.E. et al. Rumex acetosella Inhibits Platelet Function via Impaired MAPK and Phosphoinositide 3-Kinase Signaling. Chin. J. Integr. Med. 28, 802–808 (2022). https://doi.org/10.1007/s11655-021-2873-0

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  • DOI: https://doi.org/10.1007/s11655-021-2873-0

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