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Cytotoxicity assay using a human pluripotent stem cell–derived cranial neural crest cell model

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Abstract

Cleft lip and palate are the most common congenital abnormalities that occur early in pregnancy. The majority of cranial mesenchyme is derived from cranial neural crest cells that differentiate into odontoblasts, cartilage, craniofacial bone, and connective tissue. A subset of these cells differentiates into cranial ganglia. We have previously reported an induction protocol of cranial neural crest cell-like cells from human pluripotent stem cells. This study tested detection of the cytotoxic sensitivities of dental materials, including titanium ions, palladium ions, and hydroxyethyl methacrylate, on the cell viability of induced cranial neural crest cell-like cells (iNC-LCs) derived from Tic human induced pluripotent stem cell (hiPSC) line. Further, the sensitivity was compared with those of human fetal lung fibroblastic cell line MRC-5, which is origin of Tic hiPSC, and osteoblastic cell line MC3T3-E1 which was derived from mouse calvaria. The results suggested that this cell-based assay system using iNC-LCs is a potential method for in vitro screening as an alternative to animal testing to predict toxic effects of dental materials on early craniofacial development.

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Funding

This study was partially supported by grants-in-aid from the Ministry of Health, Labour and Welfare of Japan, Japan Agency for Medical Research and Development to M.K.F. (JP17bk0104011h0005), the Ministry of Education, Culture, Sports, Science and Technology of Japan to M.K.F. and M.S. (KAKENHI 15K11035); Y.M, M.S., H.N., and M.K.F (16H05535); and Y.M (17K17181).

Author information

Author notes

  1. Yuichi Mine and Mika Suga contributed equally to this work.

Authors and Affiliations

  1. Department of Medical System Engineering, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan

    Yuichi Mine, Memori Minoda & Takeshi Murayama

  2. Translational Research Center, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan

    Yuichi Mine

  3. Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition, 7-6-8, Saito-Asagi, Ibaraki, 567-0085, Osaka, Japan

    Mika Suga & Miho K. Furue

  4. iPSC-based Drug Discovery and Development Team, RIKEN BioResource Research Center, 1-7 Hikaridai, Seika-cho, Soraku-gun, 619-0237, Kyoto, Japan

    Mika Suga

  5. Department of Oral Biology and Engineering, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan

    Sumiyo Mimura & Hiroki Nikawa

Authors
  1. Yuichi Mine
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  2. Mika Suga
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  3. Sumiyo Mimura
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  5. Takeshi Murayama
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  6. Hiroki Nikawa
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  7. Miho K. Furue
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Corresponding author

Correspondence to Yuichi Mine.

Ethics declarations

Human iPSCs were used after approval by the institutional ethics review board at the National Institutes of Biomedical Innovation, Health and Nutrition (Approval number: iPS-3).

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Editor: Tetsuji Okamoto

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Mine, Y., Suga, M., Mimura, S. et al. Cytotoxicity assay using a human pluripotent stem cell–derived cranial neural crest cell model. In Vitro Cell.Dev.Biol.-Animal 56, 505–510 (2020). https://doi.org/10.1007/s11626-020-00491-0

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  • DOI: https://doi.org/10.1007/s11626-020-00491-0

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