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Icariin Ameliorates D-galactose-induced Cell Injury in Neuron-like PC12 Cells by Inhibiting MPTP Opening

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Abstract

Objective

Icariin (ICA) has a good neuroprotective effect and can upregulate neuronal basal autophagy in naturally aging rats. Mitochondrial dysfunction is associated with brain aging-related neurodegenerative diseases. Abnormal opening of the mitochondrial permeability transition pore (mPTP) is a crucial factor in mitochondrial dysfunction and is associated with excessive autophagy. This study aimed to explore that ICA protects against neuronal injury by blocking the mPTP opening and down-regulating autophagy levels in a D-galactose (D-gal)-induced cell injury model.

Methods

A cell model of neuronal injury was established in rat pheochromocytoma cells (PC12 cells) treated with 200 mmol/L D-gal for 48 h. In this cell model, PC12 cells were pre-treated with different concentrations of ICA for 24 h. MTT was used to detect cell viability. Senescence associated β-galactosidase (SA-β-Gal) staining was used to observe cell senescence. Western blot analysis was performed to detect the expression levels of a senescence-related protein (p21), autophagy markers (LC3B, p62, Atg7, Atg5 and Beclin 1), mitochondrial fission and fusion-related proteins (Drp1, Mfn2 and Opa1), and mitophagy markers (Pink1 and Parkin). The changes of autophagic flow were detected by using mRFP-GFP-LC3 adenovirus. The intracellular ultrastructure was observed by transmission electron microscopy. Immunofluorescence was used to detect mPTP, mitochondrial membrane potential (MMP), mitochondrial reactive oxygen species (mtROS) and ROS levels. ROS and apoptosis levels were detected by flow cytometry.

Results

D-gal treatment significantly decreased the viability of PC12 cells, and markedly increased the SA-β-Gal positive cells as compared to the control group. With the D-gal stimulation, the expression of p21 was significantly up-regulated. Furthermore, D-gal stimulation resulted in an elevated LC3B II/I ratio and decreased p62 expression. Meanwhile, autophagosomes and autolysosomes were significantly increased, indicating abnormal activation of autophagy levels. In addition, in this D-gal-induced model of cell injury, the mPTP was abnormally open, the ROS generation was continuously increased, the MMP was gradually decreased, and the apoptosis was increased. ICA effectively improved mitochondrial dysfunction to protect against D-gal-induced cell injury and apoptosis. It strongly inhibited excessive autophagy by blocking the opening of the mPTP. Cotreatment with ICA and an mPTP inhibitor (cyclosporin A) did not ameliorate mitochondrial dysfunction. However, the protective effects were attenuated by cotreatment with ICA and an mPTP activator (lonidamine).

Conclusion

ICA inhibits the activation of excessive autophagy and thus improves mitochondrial dysfunction by blocking the mPTP opening.

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Author notes

  1. Both authors contributed equally to this work.

Authors and Affiliations

  1. Third-grade Pharmacological Laboratory of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, China Three Gorges University, Yichang, 443002, China

    Shan-shan Hu, Tong-yao Wang, Lu Ni, Fan-xin Hu, Bo-wen Yue, Ying Zheng, Tian-lun Wang, Abhishek Kumar, Yan-yan Wang, **-e Wang & Zhi-yong Zhou

  2. College of Medicine and Health Sciences, China Three Gorges University, Yichang, 443002, China

    Shan-shan Hu, Tong-yao Wang, Lu Ni, Fan-xin Hu, Bo-wen Yue, Ying Zheng, Tian-lun Wang, Abhishek Kumar, Yan-yan Wang & Zhi-yong Zhou

  3. College of Basic Medical Science, China Three Gorges University, Yichang, 443002, China

    **-e Wang

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  1. Shan-shan Hu
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  2. Tong-yao Wang
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  3. Lu Ni
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  4. Fan-xin Hu
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  9. Yan-yan Wang
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  10. **-e Wang
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Correspondence to **-e Wang or Zhi-yong Zhou.

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The authors declare that there are no conflicts of interest.

Additional information

This study was supported by the Natural Science Foundation of Yichang City of China (No. A23-1-075).

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Hu, Ss., Wang, Ty., Ni, L. et al. Icariin Ameliorates D-galactose-induced Cell Injury in Neuron-like PC12 Cells by Inhibiting MPTP Opening. CURR MED SCI (2024). https://doi.org/10.1007/s11596-024-2892-0

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  • DOI: https://doi.org/10.1007/s11596-024-2892-0

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