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First Detection, Identification and Characterisation of Dickeya solani-Caused Potato Soft Rot in Algeria

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Abstract

Pectinolytic bacteria were isolated from potato plants and tubers showing blackleg and soft rot symptoms, collected from fields and storages in the province of Ain Defla, Algeria, in the period from 2016 to 2018. Twelve Gram-negative pectinolytic bacterial isolates were obtained from the crystal violet pectate medium growth. These strains induced a hypersensitive reaction and soft rot symptoms when inoculated in tobacco leaves and potato tuber slices, respectively. Biochemical characterisation has revealed that the isolates correspond to two distinct phenotypes. Molecular identification by PCR using specific Dickeya spp. ADE1/ADE2 primers proved that four isolates S441, B74, D14 and A13 were of the genus Dickeya. Subsequently, additional biochemical tests have allowed their classification to the Dickeya biovar 3, known as the Dickeya solani species. Pathogenicity tests performed on potato, tomato and eggplant plants confirmed their virulence in various Solanaceae. Finally, the effects of initial inoculum concentration and of various temperature and humidity regimes were investigated. Maceration assays performed on potato tuber discs revealed more severe damage at high concentrations and when increasing temperature and humidity. This first study of D. solani causing blackleg and soft rot on potato in Algeria highlights the potential risks associated with this disease in the next years.

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Acknowledgements

The authors are grateful to Dr Rey Pascal for valuable analyses of the article.

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Authors and Affiliations

  1. Protection and Development of Agro-Biological Resources Laboratory, Faculty of Nature and Life Science, Blida 1 University, Blida, Algeria

    Djamila Marok-Alim

  2. Department of Agricultural Sciences, Faculty of Nature and Life Sciences and Earth Sciences, University Djilali Bounàama of Khemis Miliana, Khemis Miliana, Algeria

    Djamila Marok-Alim & Mohamed Amine Marok

  3. Blida 1 University, Blida, Algeria

    Zoulikha Krimi

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  1. Djamila Marok-Alim
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Marok-Alim, D., Marok, M.A. & Krimi, Z. First Detection, Identification and Characterisation of Dickeya solani-Caused Potato Soft Rot in Algeria. Potato Res. 67, 495–514 (2024). https://doi.org/10.1007/s11540-023-09645-5

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