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Ethylene-induced nitric oxide production and stomatal closure in Arabidopsis thaliana depending on changes in cytosolic pH

  • Article
  • Plant Physiology
  • Published:
Chinese Science Bulletin

Abstract

We investigated changes in cytosolic pH and nitric oxide (NO) during ethylene-induced stomatal closure in Arabidopsis thaliana using pharmacological, laser scanning confocal microscopy (LSCM), and spectrophotography techniques. Treatment with ethephon (a direct source of ethylene when applied to plants) and 1-aminocycloaminopropane-1-carboxylic acid (ACC, an ethylene precursor) resulted in a rapid accumulation of NO and cytosolic alkalinization in guard cells. Acetic acid (a weak acid) and sodium orthovanadate (NaVO3; a plasmalemma H+-ATPase inhibitor) reduced stomatal closure induced by ethylene and blocked ethylene-induced activity of nitrate reductase. However, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), a NO scavenger, had no effect. These results suggest that NO production is downstream of the rise in cytosolic pH in A. thaliana.

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Authors and Affiliations

  1. Life Sciences College, Qingdao Agricultural University, Qingdao, 266109, China

    **g Liu, GuoHua Liu, Li**a Hou & **n Liu

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  1. **g Liu
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  2. GuoHua Liu
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  3. Li**a Hou
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  4. **n Liu
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Correspondence to **n Liu.

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Liu, J., Liu, G., Hou, L. et al. Ethylene-induced nitric oxide production and stomatal closure in Arabidopsis thaliana depending on changes in cytosolic pH. Chin. Sci. Bull. 55, 2403–2409 (2010). https://doi.org/10.1007/s11434-010-4033-3

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  • DOI: https://doi.org/10.1007/s11434-010-4033-3

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