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Repair effect of diabetic ulcers with recombinant human epidermal growth factor loaded by sustained-release microspheres

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Abstract

In this study the w/o/w extraction-evaporation technique was adopted to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres loading recombinant human epidermal growth factor (rhEGF). The microspheres were characterized for morphology by transmission electron microscopy (TEM) and particle size distribution. The release performances, the proliferation effects and therapeutic effects of rhEGF-loaded PLGA microspheres were all studied. The results showed that these spherical microspheres had a narrow size distribution and a high drug encapsulation efficiency (85.6%). RhEGF-loaded microspheres enhanced the growth rate of fibroblasts and wound healing more efficiently than pure rhEGF. The number of the proliferating cell nuclear antigen (PCNA) in the epidermis layer with the microsphere treatment was significantly larger than those of the control groups. Overall locally sustained delivery of rhEGF from biodegradable PLGA microspheres may serve as a novel therapeutic strategy for diabetic ulcer repair.

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Authors and Affiliations

  1. Institute of Nanobiotechnology, School of Materials Science and Engineering, Tian** University, Tian**, 300072, China

    **aoQing Dong, WeiCai Wang, Hao Luo, **aoFei Liang, Lei Zhang, HanJie Wang & ** Chang

  2. Department of Diabetic Foot, Tian** Medical University Metabolic Disease Hospital, Tian**, 300070, China

    Jun Xu & PengHua Wang

Authors
  1. **aoQing Dong
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  2. Jun Xu
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  3. WeiCai Wang
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  4. Hao Luo
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  5. **aoFei Liang
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  6. Lei Zhang
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  7. HanJie Wang
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  8. PengHua Wang
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  9. ** Chang
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Correspondence to ** Chang.

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Supported by the National Natural Science Foundation of China (Grant No. 50373033), the Applied Foundational Research Key Fund of Tian** (Grant No. 05YFJZJC01001) and the International Cooperative Fund of Tian** (Grant No. 05YFGHHZ20070)

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Dong, X., Xu, J., Wang, W. et al. Repair effect of diabetic ulcers with recombinant human epidermal growth factor loaded by sustained-release microspheres. SCI CHINA SER C 51, 1039–1044 (2008). https://doi.org/10.1007/s11427-008-0126-5

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  • DOI: https://doi.org/10.1007/s11427-008-0126-5

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