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Templated freezing assembly precisely regulates molecular assembly for free-standing centimeter-scale microtextured nanofilms

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Abstract

Nature provides diverse models for manufacturing complex and hierarchical materials by controlling molecular assembly at scales ranging from sub-nano to macroscale. However, develo** artificial strategies for manufacturing hierarchical materials with comparable machining capabilities to nature is extremely challenging. Here, a templated freezing assembly strategy is reported, enabling simultaneously regulating molecular assembly spatiotemporally to obtain hierarchical materials with structure control from sub-nano to macroscale. In this way, unique centimeter-scale freestanding nanofilms are assembled from diverse molecules, e.g., proteins and conjugated polymers. A generated silk fibroin (SF) nanofilm presents a tunable β-sheet fraction from 5% to 47%, fiber width from 30 to 3,000 nm, and micro-textures with desired shapes. Such a strategy will lay the foundation for customizing hierarchical functional materials from single or multi-component molecules, e.g., desired bio-scaffolds with controlled cell adhesion.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2020YFE0100300, 2018YFA0208502), the National Natural Science Foundation of China (51925307, 21733010, 32001083, 22105210), the Key Research Program of Frontier Sciences, Chinese Academy of Sciences (ZDBS-LY-SLH031) and the Bei**g National Laboratory for Molecular Sciences (BNLMS-CXXM-2020BMS20025). X. Z. thanks the Strategic Priority Research Program, Chinese Academy of Sciences (XDB28000000) and the National Natural Science Foundation of China (12174388). Authors thank Prof. Lanqun Mao (College of Chemistry, Bei**g Normal University) and Prof. Ming Wang (Institute of Chemistry, Chinese Academy of Sciences, ICCAS) for providing cells and their guidance in biology. We thank Center for Physico-chemical Analysis and Measurement, ICCAS, for assisting the cryo-TEM SAED data collection.

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Authors and Affiliations

  1. Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Bei**g, 100190, China

    Junqiang Mao, Huimei Cao, Jie Liu, Qingrui Fan & Jianjun Wang

  2. School of Chemical Sciences, University of Chinese Academy of Sciences, Bei**g, 100049, China

    Junqiang Mao & Huimei Cao

  3. School of Physical Sciences and CAS Center for Excellence in Topological Quantum Computation, University of Chinese Academy of Sciences, Bei**g, 100049, China

    **n Zhou

  4. School of Future Technology, University of Chinese Academy of Sciences, Bei**g, 100049, China

    Jianjun Wang

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Correspondence to **n Zhou, Qingrui Fan or Jianjun Wang.

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Supporting information The supporting information is available online at https://chem.scichina.com and https://springer.longhoe.net/journal/11426. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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Templated freezing assembly precisely regulates molecular assembly for free-standing centimeter-scale microtextured nanofilms

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Mao, J., Cao, H., Liu, J. et al. Templated freezing assembly precisely regulates molecular assembly for free-standing centimeter-scale microtextured nanofilms. Sci. China Chem. 66, 878–886 (2023). https://doi.org/10.1007/s11426-022-1476-y

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