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Inhibitory effects of juglanin on cellular senescence in human dermal fibroblasts

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Abstract

Cellular senescence contributes to tissue and organismal aging, tumor suppression and progress, tissue repair and regeneration, and age-related diseases. Thus, aging intervention might be a promising target for treatment and prevention of diverse age-related diseases. In the present study, we investigated whether juglanin purified from the crude extract of Polygonum aviculare exerted inhibitory activity against cellular senescence in human dermal fibroblasts (HDFs). Juglanin decreased senescence-associated β-galactosidase activity (SA-β-gal) and the level of reactive oxygen species in senescent cells induced by adriamycin treatment. Juglanin also repressed SA-β-gal activity in HDFs under replicative senescence. These results suggest that juglanin represses cellular senescence in HDFs and might be useful for the development of dietary supplements or cosmetics that alleviate tissue aging or age-related diseases.

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Abbreviations

HDF:

Human dermal fibroblast

HUVEC:

Human umbilical vein endothelial cell

EGM-2:

Endothelial cell growth medium-2

SA-β-gal:

Senescence-associated β-galactosidase

mTOR:

Mammalian target of rapamycin

X-gal:

5-Bromo-4-chloro-3-indolyl-β-d-galactopyranoside

PD:

Population doubling

DMEM:

Dulbecco’s modified Eagle medium

H2DCFDA:

2′,7′-Dichlorodihydrofluorescein diacetate

DMSO:

Dimethyl sulfoxide

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Acknowledgments

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2005-0049417).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University, 317-1 Daemyung-Dong, Daegu, 705-717, Republic of Korea

    Hyo Hyun Yang, Hwa Young Kim, Suk Hwan Baek & Jae-Ryong Kim

  2. Department of Physiology, College of Medicine, Yeungnam University, Daegu, 705-717, Republic of Korea

    So Young Park

  3. Department of Pharmacology, College of Medicine, Yeungnam University, Daegu, 705-717, Republic of Korea

    Hyung Chul Choi

  4. Aging-associated Vascular Disease Research Center, College of Medicine, Yeungnam University, Daegu, 705-717, Republic of Korea

    Jong-Keun Son, Hwa Young Kim, Suk Hwan Baek, Hyung Chul Choi, So Young Park & Jae-Ryong Kim

  5. College of Pharmacy, Yeungnam University, Gyongsan, 712-749, Republic of Korea

    Kyoung Hwangbo & Jong-Keun Son

  6. College of Pharmacy, Yanbian University, Yanji, 133000, People’s Republic of China

    Ming Shan Zheng

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  1. Hyo Hyun Yang
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Correspondence to Jae-Ryong Kim.

Additional information

H. H. Yang, K. Hwangbo and M. S. Zheng contributed equally to this work.

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Yang, H.H., Hwangbo, K., Zheng, M.S. et al. Inhibitory effects of juglanin on cellular senescence in human dermal fibroblasts. J Nat Med 68, 473–480 (2014). https://doi.org/10.1007/s11418-014-0817-0

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  • DOI: https://doi.org/10.1007/s11418-014-0817-0

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