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Morphological and biochemical responses of Vicia faba (faba beans) grown on fly ash amended soil in the presence of Rhizobium leguminosarum and arbuscular mycorrhizal fungus

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Abstract

An experiment was conducted in the greenhouse to investigate the feasibility of Vicia faba grown on different fly ash concentrations (0–30%) and dual inoculation with Rhizobium and arbuscular mycorrhizal fungi (AMF). Sampling was done 45 days after sowing to analyse the plant growth parameters, photosynthetic attributes (total chlorophyll and carotenoids content), protein content, nitrogen (N) and phosphorus (P) content, defensive factors (antioxidant activity and proline content) and damage markers (lipid peroxidation, reactive oxygen species and cell viability). The results revealed that the application of fly ash (FA) alone did not result in any significant improvement in growth, biochemical and physiological parameters. However, dual inoculation showed a synergistic impact on legume growth, photosynthetic pigments, protein, proline, and cell viability. Rhizobium, AMF and 10% FA showed maximum enhancement in all attributes mentioned. 20% and 30% fly doses showed a reduction in growth, photosynthesis and antioxidants and caused oxidative stress via lipid peroxidation. The results showed that the synergistic or combined interactions between all three variables of the symbiotic relationship (Rhizobium-legume-AMF) boosted plant productivity.

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Acknowledgements

The authors are highly thankful to the Chairperson of Department of Botany, Aligarh Muslim University, India for providing the required facilities and assistance to carry out the experimental work. The author also acknowledges University Grants Commission for giving (UGC Non-NET) Fellowship and USIF, AMU for providing the SEM facility.

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  1. Environmental Physiology Laboratory, Department of Botany, Aligarh Muslim University, U.P, Aligarh, 202002, India

    Alisha Hussain, Shahla Faizan, Rinkee Kumari & Ekta Pandey

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Alisha Hussain: Investigation, methodology, formal analysis & writing. Shahla faizan: Editing & reviewing. Rinkee kumari and Ekta Pandey: Reviewing.

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Hussain, A., Faizan, S., Kumari, R. et al. Morphological and biochemical responses of Vicia faba (faba beans) grown on fly ash amended soil in the presence of Rhizobium leguminosarum and arbuscular mycorrhizal fungus. Environ Sci Pollut Res (2024). https://doi.org/10.1007/s11356-024-34154-4

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