Abstract
The present study aimed to report the morphometric and hematological indices and genotoxicity of a free-life population of D’Orbigny’s slider turtles (Trachemys dorbigni) living in an urban area in Southern Brazil. For that, 16 specimens were randomly captured in an urban canal that receives irregular releases of wastewater. Biometrics and external visual changes were analyzed, such as turtle shell deformities, and the presence of parasites. Blood samples were collected to evaluate the hematological profile and the presence of micronuclei and other erythrocyte nuclear abnormalities as potential mutagenic and genotoxic effects. Water physicochemical parameters were also measured. Organisms with ectoparasites (31.25%) and small carapace deformations (56.25%) were observed, but maximum carapace length and weight were considered normal for the species according to the literature. The blood profile indicated low hemoglobin and hematocrit and a high number of total leukocytes, particularly eosinophils which characterize parasitic infections. A frequency of 0.12% for the micronucleus was considered basal, but the frequency of other erythrocyte abnormalities was evident, mainly of blebbed nuclei (63.79%), indicating chromosomal damage in the early stage. The results of this study suggest that natural populations of chelonian inhabiting urbanized areas are impacted by anthropogenic activities in the surrounding environment. Furthermore, it provides comprehensive data which can serve as a comparative model for environmental monitoring studies involving turtles.
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Data availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We thank Katiuscia Bueno and Gabriel Zani for their help in capturing the animals and collecting samples and to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES-001 for the Danielle Tavares da Silva fellowship.
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This work was supported by the Coordination for the Improvement of Higher Education Personnel—CAPES. DL and CMGM acknowledge the financial support from CNPq (Productivity grants PQ 310859/2020–4 and PQ 312898/2020–7, respectively).
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Daniel Loebmann and Camila De Martinez Gaspar Martins conceived the study and were responsible for the conception and general planning, obtaining financial support and managing it, as well as for the revision. Danielle Tavares da Silva conceived the study, collected samples and analyzed them, interpreted the data, and wrote de work. Raqueli Teresinha Franca processed the analysis of hematological parameters. Thays de Andrade Guedes performed the micronucleus test. The first version of the manuscript was written by Danielle Tavares da Silva, and all authors commented on previous versions of the manuscript, providing feedback and hel** to shape the research.
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da Silva, D.T., Guedes, T.d.A., França, R.T. et al. Hematological and genotoxic biomarkers in a natural population of freshwater turtles Trachemys dorbigni (Duméril & Bibron, 1835) (Testudines: Emydidae) living in an urban area in Southern Brazil. Environ Sci Pollut Res (2024). https://doi.org/10.1007/s11356-024-33515-3
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DOI: https://doi.org/10.1007/s11356-024-33515-3