Abstract
In this study, a laboratory-scale partial nitrification reactor (PN reactor) was used to treat high-ammonia–nitrogen wastewater, by changing the influent NH4+-N conversion rate as the main operating strategy, to investigate the upper limit of its NH4+-N conversion rate (ACR) and explore its feasibility as an anammox pre-process. During the experiment, PN reactor was successfully activated in only 10 days. The PN reactor reached the highest ACR value of approximately 10.24 kg N/(m3 · day) when the influent ACR was 16.57 kg N/(m3 · day), and the ammonia conversion efficiency (ACE) was 61.78% at this time. The ratio of [NO2−-N]Eff/[NH4+-N]Eff was approximately 1.37 which was close to the theoretical ratio of 1.32. And feasibility exploration experiment proved that it was feasible to use this PN reactor as a pre-process of anammox. The PCR-DGGE results showed that the dominant phylum and genus in the reactor during the ACR experiment were Proteobacteria and Nitrosomonas, respectively. With the increase in the ACR, the relative concentration of Nitrosomonas sp. G1 increased from 15 to 40%. This indicates that its abundance is directly correlated with the increase in the ACR. High-throughput sequencing showed that increasing the ACR of the PN reactor greatly reduced the diversity and abundance of the system microbial community structure and changed the dominant phylum and genus; however, the stability of the system was not disrupted. High-throughput sequencing experiments showed that the abundance value of nitrosation enzymes accounted for 91.62%, which was positively correlated with the expression of nitrification genes in the genus Nitrosomonas.
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This research was funded by the Guangxi Natural Science Foundation, grant number 2019GXNSFFA245017.
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Material preparation, data collection, and analysis were performed by Yue **. The first draft of the manuscript was written by Yue ** and Xuli Zhang. Prof. Haixiang Li directed the experiment plan and data analysis; Zhicheng Wu set up the experimental reactor and carried out the data collection; the manuscript revision and submission were completed by Wenjie Zhang. And all authors contributed to the study conception and design; all authors commented on previous versions of the manuscript, read, and approved the final manuscript.
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**, Y., Zhang, X., Li, H. et al. High-rate partial nitritation as a pretreatment of anammox process. Environ Sci Pollut Res 30, 104592–104602 (2023). https://doi.org/10.1007/s11356-023-29663-7
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DOI: https://doi.org/10.1007/s11356-023-29663-7